Mascot Search Results

Peptide View

MS/MS Fragmentation of NSNSYGIPEPAHAYAQPQTTTPLPAVSGSPGAAITPLPSTQNGPVFAK
Found in ENSP00000346300, gene:CRKL -- Crk-like protein. [Source:Uniprot/SWISSPROT;Acc:P46109]

Match to Query 9584: 4881.378300 from(1221.351851,4+)
Title: Elution from: 777.777 to 777.777 period: 070608_eb1_light_10min_ip2.raw experiment: 1 cycles: 1 precIntensity: 32630.0 FinneganScanNumber: 7958
Data file 070608_eb1_light_10min_conj.msm

Monoisotopic mass of neutral peptide Mr(calc): 4881.3650
Fixed modifications: Carbamidomethyl (C)
Variable modifications: 
Y14    : Phospho (Y)
Ions Score: 63  Expect: 0.0024  
Matches (Bold Red): 60/546 fragment ions using 59 most intense peaks

#	b	b⁺⁺	b*	b*⁺⁺	b⁰	b⁰⁺⁺	Seq.	y	y⁺⁺	y*	y*⁺⁺	y⁰	y⁰⁺⁺	#
1	115.0502	58.0287	98.0237	49.5155			N							48
2	202.0822	101.5448	185.0557	93.0315	184.0717	92.5395	S	4768.3294	2384.6683	4751.3028	2376.1550	4750.3188	2375.6630	47
3	316.1252	158.5662	299.0986	150.0529	298.1146	149.5609	N	4681.2973	2341.1523	4664.2708	2332.6390	4663.2868	2332.1470	46
4	403.1572	202.0822	386.1306	193.5690	385.1466	193.0770	S	4567.2544	2284.1308	4550.2278	2275.6176	4549.2438	2275.1256	45
5	566.2205	283.6139	549.1940	275.1006	548.2100	274.6086	Y	4480.2224	2240.6148	4463.1958	2232.1015	4462.2118	2231.6095	44
6	623.2420	312.1246	606.2154	303.6114	605.2314	303.1193	G	4317.1590	2159.0832	4300.1325	2150.5699	4299.1485	2150.0779	43
7	736.3260	368.6667	719.2995	360.1534	718.3155	359.6614	I	4260.1376	2130.5724	4243.1110	2122.0592	4242.1270	2121.5671	42
8	833.3788	417.1930	816.3523	408.6798	815.3682	408.1878	P	4147.0535	2074.0304	4130.0270	2065.5171	4129.0430	2065.0251	41
9	962.4214	481.7143	945.3948	473.2011	944.4108	472.7091	E	4050.0008	2025.5040	4032.9742	2016.9907	4031.9902	2016.4987	40
10	1059.4742	530.2407	1042.4476	521.7274	1041.4636	521.2354	P	3920.9582	1960.9827	3903.9316	1952.4694	3902.9476	1951.9774	39
11	1130.5113	565.7593	1113.4847	557.2460	1112.5007	556.7540	A	3823.9054	1912.4563	3806.8789	1903.9431	3805.8948	1903.4511	38
12	1267.5702	634.2887	1250.5436	625.7755	1249.5596	625.2834	H	3752.8683	1876.9378	3735.8417	1868.4245	3734.8577	1867.9325	37
13	1338.6073	669.8073	1321.5807	661.2940	1320.5967	660.8020	A	3615.8094	1808.4083	3598.7828	1799.8951	3597.7988	1799.4030	36
14	1581.6369	791.3221	1564.6104	782.8088	1563.6264	782.3168	Y	3544.7723	1772.8898	3527.7457	1764.3765	3526.7617	1763.8845	35
15	1652.6741	826.8407	1635.6475	818.3274	1634.6635	817.8354	A	3301.7426	1651.3749	3284.7161	1642.8617	3283.7321	1642.3697	34
16	1780.7326	890.8700	1763.7061	882.3567	1762.7221	881.8647	Q	3230.7055	1615.8564	3213.6790	1607.3431	3212.6949	1606.8511	33
17	1877.7854	939.3963	1860.7588	930.8831	1859.7748	930.3911	P	3102.6469	1551.8271	3085.6204	1543.3138	3084.6364	1542.8218	32
18	2005.8440	1003.4256	1988.8174	994.9123	1987.8334	994.4203	Q	3005.5942	1503.3007	2988.5676	1494.7874	2987.5836	1494.2954	31
19	2106.8917	1053.9495	2089.8651	1045.4362	2088.8811	1044.9442	T	2877.5356	1439.2714	2860.5090	1430.7582	2859.5250	1430.2662	30
20	2207.9393	1104.4733	2190.9128	1095.9600	2189.9288	1095.4680	T	2776.4879	1388.7476	2759.4614	1380.2343	2758.4773	1379.7423	29
21	2308.9870	1154.9971	2291.9605	1146.4839	2290.9764	1145.9919	T	2675.4402	1338.2238	2658.4137	1329.7105	2657.4297	1329.2185	28
22	2406.0398	1203.5235	2389.0132	1195.0102	2388.0292	1194.5182	P	2574.3926	1287.6999	2557.3660	1279.1866	2556.3820	1278.6946	27
23	2519.1238	1260.0656	2502.0973	1251.5523	2501.1133	1251.0603	L	2477.3398	1239.1735	2460.3132	1230.6603	2459.3292	1230.1683	26
24	2616.1766	1308.5919	2599.1500	1300.0787	2598.1660	1299.5867	P	2364.2557	1182.6315	2347.2292	1174.1182	2346.2452	1173.6262	25
25	2687.2137	1344.1105	2670.1872	1335.5972	2669.2031	1335.1052	A	2267.2030	1134.1051	2250.1764	1125.5918	2249.1924	1125.0998	24
26	2786.2821	1393.6447	2769.2556	1385.1314	2768.2715	1384.6394	V	2196.1659	1098.5866	2179.1393	1090.0733	2178.1553	1089.5813	23
27	2873.3141	1437.1607	2856.2876	1428.6474	2855.3036	1428.1554	S	2097.0975	1049.0524	2080.0709	1040.5391	2079.0869	1040.0471	22
28	2930.3356	1465.6714	2913.3091	1457.1582	2912.3250	1456.6662	G	2010.0654	1005.5363	1993.0389	997.0231	1992.0549	996.5311	21
29	3017.3676	1509.1875	3000.3411	1500.6742	2999.3571	1500.1822	S	1953.0440	977.0256	1936.0174	968.5123	1935.0334	968.0203	20
30	3114.4204	1557.7138	3097.3938	1549.2006	3096.4098	1548.7086	P	1866.0119	933.5096	1848.9854	924.9963	1848.0014	924.5043	19
31	3171.4419	1586.2246	3154.4153	1577.7113	3153.4313	1577.2193	G	1768.9592	884.9832	1751.9326	876.4699	1750.9486	875.9779	18
32	3242.4790	1621.7431	3225.4524	1613.2298	3224.4684	1612.7378	A	1711.9377	856.4725	1694.9112	847.9592	1693.9271	847.4672	17
33	3313.5161	1657.2617	3296.4895	1648.7484	3295.5055	1648.2564	A	1640.9006	820.9539	1623.8741	812.4407	1622.8900	811.9487	16
34	3426.6001	1713.8037	3409.5736	1705.2904	3408.5896	1704.7984	I	1569.8635	785.4354	1552.8369	776.9221	1551.8529	776.4301	15
35	3527.6478	1764.3275	3510.6213	1755.8143	3509.6372	1755.3223	T	1456.7794	728.8934	1439.7529	720.3801	1438.7689	719.8881	14
36	3624.7006	1812.8539	3607.6740	1804.3406	3606.6900	1803.8486	P	1355.7318	678.3695	1338.7052	669.8562	1337.7212	669.3642	13
37	3737.7846	1869.3960	3720.7581	1860.8827	3719.7741	1860.3907	L	1258.6790	629.8431	1241.6524	621.3299	1240.6684	620.8379	12
38	3834.8374	1917.9223	3817.8108	1909.4091	3816.8268	1908.9171	P	1145.5949	573.3011	1128.5684	564.7878	1127.5844	564.2958	11
39	3921.8694	1961.4383	3904.8429	1952.9251	3903.8589	1952.4331	S	1048.5422	524.7747	1031.5156	516.2614	1030.5316	515.7694	10
40	4022.9171	2011.9622	4005.8906	2003.4489	4004.9065	2002.9569	T	961.5101	481.2587	944.4836	472.7454	943.4996	472.2534	9
41	4150.9757	2075.9915	4133.9491	2067.4782	4132.9651	2066.9862	Q	860.4625	430.7349	843.4359	422.2216			8
42	4265.0186	2133.0129	4247.9921	2124.4997	4247.0080	2124.0077	N	732.4039	366.7056	715.3773	358.1923			7
43	4322.0401	2161.5237	4305.0135	2153.0104	4304.0295	2152.5184	G	618.3610	309.6841	601.3344	301.1708			6
44	4419.0928	2210.0501	4402.0663	2201.5368	4401.0823	2201.0448	P	561.3395	281.1734	544.3129	272.6601			5
45	4518.1612	2259.5843	4501.1347	2251.0710	4500.1507	2250.5790	V	464.2867	232.6470	447.2602	224.1337			4
46	4665.2297	2333.1185	4648.2031	2324.6052	4647.2191	2324.1132	F	365.2183	183.1128	348.1918	174.5995			3
47	4736.2668	2368.6370	4719.2402	2360.1237	4718.2562	2359.6317	A	218.1499	109.5786	201.1234	101.0653			2
48							K	147.1128	74.0600	130.0863	65.5468			1

Error Distribution Error Distribution (ppm)

NCBI BLAST search of NSNSYGIPEPAHAYAQPQTTTPLPAVSGSPGAAITPLPSTQNGPVFAK
(Parameters: blastp, nr protein database, expect=20000, no filter, PAM30)
Other BLAST web gateways

All matches to this query

Score	Mr(calc):	Delta	Sequence
63.0	4881.3650	0.0133	NSNSYGIPEPAHAYAQPQTTTPLPAVSGSPGAAITPLPSTQNGPVFAK
53.0	4881.3650	0.0133	NSNSYGIPEPAHAYAQPQTTTPLPAVSGSPGAAITPLPSTQNGPVFAK
23.8	4881.3650	0.0133	NSNSYGIPEPAHAYAQPQTTTPLPAVSGSPGAAITPLPSTQNGPVFAK
23.7	4881.3650	0.0133	NSNSYGIPEPAHAYAQPQTTTPLPAVSGSPGAAITPLPSTQNGPVFAK
22.3	4881.3650	0.0133	NSNSYGIPEPAHAYAQPQTTTPLPAVSGSPGAAITPLPSTQNGPVFAK
21.0	4881.3650	0.0133	NSNSYGIPEPAHAYAQPQTTTPLPAVSGSPGAAITPLPSTQNGPVFAK
21.0	4881.3650	0.0133	NSNSYGIPEPAHAYAQPQTTTPLPAVSGSPGAAITPLPSTQNGPVFAK
20.9	4881.3650	0.0133	NSNSYGIPEPAHAYAQPQTTTPLPAVSGSPGAAITPLPSTQNGPVFAK
20.9	4881.3650	0.0133	NSNSYGIPEPAHAYAQPQTTTPLPAVSGSPGAAITPLPSTQNGPVFAK
19.8	4881.3650	0.0133	NSNSYGIPEPAHAYAQPQTTTPLPAVSGSPGAAITPLPSTQNGPVFAK

Mascot: http://www.matrixscience.com/