Mascot Search Results

Peptide View

MS/MS Fragmentation of VTIADDYSDPFDAK
Found in ENSP00000262512, gene:SHB -- SH2 domain-containing adapter protein B. [Source:Uniprot/SWISSPROT;Acc:Q15464]

Match to Query 9592: 1635.682092 from(818.848322,2+)
Title: Elution from: 777.777 to 777.777 period: 021107_dwbidira_py100_ip2.raw experiment: 1 cycles: 1 precIntensity: 40710.0 FinneganScanNumber: 8025
Data file 021107_BidirA_B2DW_10min_conj_2.msm

Monoisotopic mass of neutral peptide Mr(calc): 1635.6705
Fixed modifications: Carbamidomethyl (C)
Variable modifications: 
Y7     : Phospho (Y)
Ions Score: 50  Expect: 0.012  
Matches (Bold Red): 33/124 fragment ions using 54 most intense peaks

#	b	b⁺⁺	b⁰	b⁰⁺⁺	Seq.	y	y⁺⁺	y*	y*⁺⁺	y⁰	y⁰⁺⁺	#
1	100.0757	50.5415			V							14
2	201.1234	101.0653	183.1128	92.0600	T	1537.6094	769.3083	1520.5828	760.7951	1519.5988	760.3030	13
3	314.2074	157.6074	296.1969	148.6021	I	1436.5617	718.7845	1419.5351	710.2712	1418.5511	709.7792	12
4	385.2445	193.1259	367.2340	184.1206	A	1323.4776	662.2425	1306.4511	653.7292	1305.4671	653.2372	11
5	500.2715	250.6394	482.2609	241.6341	D	1252.4405	626.7239	1235.4140	618.2106	1234.4300	617.7186	10
6	615.2984	308.1528	597.2879	299.1476	D	1137.4136	569.2104	1120.3870	560.6972	1119.4030	560.2051	9
7	858.3281	429.6677	840.3175	420.6624	Y	1022.3866	511.6970	1005.3601	503.1837	1004.3761	502.6917	8
8	945.3601	473.1837	927.3495	464.1784	S	779.3570	390.1821	762.3304	381.6689	761.3464	381.1769	7
9	1060.3870	530.6972	1042.3765	521.6919	D	692.3250	346.6661	675.2984	338.1528	674.3144	337.6608	6
10	1157.4398	579.2235	1139.4292	570.2183	P	577.2980	289.1526	560.2715	280.6394	559.2875	280.1474	5
11	1304.5082	652.7577	1286.4976	643.7525	F	480.2453	240.6263	463.2187	232.1130	462.2347	231.6210	4
12	1419.5352	710.2712	1401.5246	701.2659	D	333.1769	167.0921	316.1503	158.5788	315.1663	158.0868	3
13	1490.5723	745.7898	1472.5617	736.7845	A	218.1499	109.5786	201.1234	101.0653			2
14					K	147.1128	74.0600	130.0863	65.5468			1

Error Distribution Error Distribution (ppm)

NCBI BLAST search of VTIADDYSDPFDAK
(Parameters: blastp, nr protein database, expect=20000, no filter, PAM30)
Other BLAST web gateways

All matches to this query

Score Mr(calc): Delta Sequence

49.9 1635.6705 0.0116 VTIADDYSDPFDAK

8.2 1635.7038 -0.0217 GASAPPQDPASSARNK

7.8 1635.6974 -0.0153 TVLAERVYDSWK

7.4 1635.7038 -0.0217 GASAPPQDPASSARNK

6.5 1635.6512 0.0309 MGPGATAGGAEKSNVK

6.2 1635.7103 -0.0282 AQQESAQAENHLNGK

5.8 1635.6775 0.0046 AVTLDKDAYYRR

5.8 1635.6775 0.0046 AVTLDKDAYYRR

5.7 1635.7103 -0.0282 AQQESAQAENHLNGK

5.4 1635.6913 -0.0092 TNSTNNHKDKNLR

Score	Mr(calc):	Delta	Sequence
49.9	1635.6705	0.0116	VTIADDYSDPFDAK
8.2	1635.7038	-0.0217	GASAPPQDPASSARNK
7.8	1635.6974	-0.0153	TVLAERVYDSWK
7.4	1635.7038	-0.0217	GASAPPQDPASSARNK
6.5	1635.6512	0.0309	MGPGATAGGAEKSNVK
6.2	1635.7103	-0.0282	AQQESAQAENHLNGK
5.8	1635.6775	0.0046	AVTLDKDAYYRR
5.8	1635.6775	0.0046	AVTLDKDAYYRR
5.7	1635.7103	-0.0282	AQQESAQAENHLNGK
5.4	1635.6913	-0.0092	TNSTNNHKDKNLR

Mascot: http://www.matrixscience.com/