Mascot Search Results

Peptide View

MS/MS Fragmentation of VTIADDYSDPFDAK
Found in ENSP00000262512, gene:SHB -- SH2 domain-containing adapter protein B. [Source:Uniprot/SWISSPROT;Acc:Q15464]

Match to Query 14673: 1637.665206 from(819.839879,2+)
Title: Elution from: 777.777 to 777.777 period: 061107_bidirb_10min_4g10_ip1.raw experiment: 1 cycles: 1 precIntensity: 59433.0 FinneganScanNumber: 7381
Data file 061107_BidirB_B2_10min_conj.msm

Monoisotopic mass of neutral peptide Mr(calc): 1637.6646
Fixed modifications: Carbamidomethyl (C)
Variable modifications: 
Y7     : Phospho (Y)
K14    : Lysine-15N2 (K_2)
Ions Score: 71  Expect: 7.6e-05  
Matches (Bold Red): 28/124 fragment ions using 36 most intense peaks

#	b	b⁺⁺	b⁰	b⁰⁺⁺	Seq.	y	y⁺⁺	y*	y*⁺⁺	y⁰	y⁰⁺⁺	#
1	100.0757	50.5415			V							14
2	201.1234	101.0653	183.1128	92.0600	T	1539.6035	770.3054	1522.5769	761.7921	1521.5929	761.3001	13
3	314.2074	157.6074	296.1969	148.6021	I	1438.5558	719.7815	1421.5292	711.2683	1420.5452	710.7762	12
4	385.2445	193.1259	367.2340	184.1206	A	1325.4717	663.2395	1308.4452	654.7262	1307.4611	654.2342	11
5	500.2715	250.6394	482.2609	241.6341	D	1254.4346	627.7209	1237.4081	619.2077	1236.4240	618.7157	10
6	615.2984	308.1528	597.2879	299.1476	D	1139.4077	570.2075	1122.3811	561.6942	1121.3971	561.2022	9
7	858.3281	429.6677	840.3175	420.6624	Y	1024.3807	512.6940	1007.3542	504.1807	1006.3702	503.6887	8
8	945.3601	473.1837	927.3495	464.1784	S	781.3511	391.1792	764.3245	382.6659	763.3405	382.1739	7
9	1060.3870	530.6972	1042.3765	521.6919	D	694.3190	347.6632	677.2925	339.1499	676.3085	338.6579	6
10	1157.4398	579.2235	1139.4292	570.2183	P	579.2921	290.1497	562.2656	281.6364	561.2815	281.1444	5
11	1304.5082	652.7577	1286.4976	643.7525	F	482.2393	241.6233	465.2128	233.1100	464.2288	232.6180	4
12	1419.5352	710.2712	1401.5246	701.2659	D	335.1709	168.0891	318.1444	159.5758	317.1604	159.0838	3
13	1490.5723	745.7898	1472.5617	736.7845	A	220.1440	110.5756	203.1174	102.0624			2
14					K	149.1069	75.0571	132.0803	66.5438			1

Error Distribution Error Distribution (ppm)

NCBI BLAST search of VTIADDYSDPFDAK
(Parameters: blastp, nr protein database, expect=20000, no filter, PAM30)
Other BLAST web gateways

All matches to this query

Score Mr(calc): Delta Sequence

71.2 1637.6646 0.0006 VTIADDYSDPFDAK

9.1 1637.6978 -0.0326 GASAPPQDPASSARNK

9.1 1637.6978 -0.0326 GASAPPQDPASSARNK

8.8 1637.6534 0.0118 MRANNQKVYMPR

7.6 1637.6978 -0.0326 GASAPPQDPASSARNK

6.1 1637.6586 0.0066 AELSSNATTSLVQR

6.1 1637.6586 0.0066 AELSSNATTSLVQR

5.3 1637.6586 0.0066 AELSSNATTSLVQR

5.3 1637.6586 0.0066 AELSSNATTSLVQR

4.4 1637.6586 0.0066 AELSSNATTSLVQR

Score	Mr(calc):	Delta	Sequence
71.2	1637.6646	0.0006	VTIADDYSDPFDAK
9.1	1637.6978	-0.0326	GASAPPQDPASSARNK
9.1	1637.6978	-0.0326	GASAPPQDPASSARNK
8.8	1637.6534	0.0118	MRANNQKVYMPR
7.6	1637.6978	-0.0326	GASAPPQDPASSARNK
6.1	1637.6586	0.0066	AELSSNATTSLVQR
6.1	1637.6586	0.0066	AELSSNATTSLVQR
5.3	1637.6586	0.0066	AELSSNATTSLVQR
5.3	1637.6586	0.0066	AELSSNATTSLVQR
4.4	1637.6586	0.0066	AELSSNATTSLVQR

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