Mascot Search Results

Peptide View

MS/MS Fragmentation of SEYSVHSSLASK
Found in ENSP00000302530, gene:BUB1 -- Mitotic checkpoint serine/threonine-protein kinase BUB1 (EC 2.7.11.1) (hBUB1) (BUB1A

Match to Query 15199: 1381.605786 from(691.810169,2+)
Title: Elution from: 777.777 to 777.777 period: 100308_b2dw_b1fc_5min_4g10_ip2.raw experiment: 1 cycles: 1 precIntensity: 17979.0 FinneganScanNumber: 3023
Data file 100308_DWB2_b1Fc_5min_conj.msm

Monoisotopic mass of neutral peptide Mr(calc): 1381.6006
Fixed modifications: Carbamidomethyl (C)
Variable modifications: 
Y3     : Phospho (Y)
K12    : Lysine-13C615N2 (K_8)
Ions Score: 50  Expect: 0.0062  
Matches (Bold Red): 18/108 fragment ions using 23 most intense peaks

#	b	b⁺⁺	b⁰	b⁰⁺⁺	Seq.	y	y⁺⁺	y*	y*⁺⁺	y⁰	y⁰⁺⁺	#
1	88.0393	44.5233	70.0287	35.5180	S							12
2	217.0819	109.0446	199.0713	100.0393	E	1295.5759	648.2916	1278.5493	639.7783	1277.5653	639.2863	11
3	460.1115	230.5594	442.1010	221.5541	Y	1166.5333	583.7703	1149.5067	575.2570	1148.5227	574.7650	10
4	547.1436	274.0754	529.1330	265.0701	S	923.5036	462.2554	906.4771	453.7422	905.4931	453.2502	9
5	646.2120	323.6096	628.2014	314.6044	V	836.4716	418.7394	819.4450	410.2262	818.4610	409.7341	8
6	783.2709	392.1391	765.2603	383.1338	H	737.4032	369.2052	720.3766	360.6920	719.3926	360.1999	7
7	870.3029	435.6551	852.2924	426.6498	S	600.3443	300.6758	583.3177	292.1625	582.3337	291.6705	6
8	957.3350	479.1711	939.3244	470.1658	S	513.3122	257.1598	496.2857	248.6465	495.3017	248.1545	5
9	1070.4190	535.7131	1052.4085	526.7079	L	426.2802	213.6437	409.2537	205.1305	408.2696	204.6385	4
10	1141.4561	571.2317	1123.4456	562.2264	A	313.1961	157.1017	296.1696	148.5884	295.1856	148.0964	3
11	1228.4882	614.7477	1210.4776	605.7424	S	242.1590	121.5832	225.1325	113.0699	224.1485	112.5779	2
12					K	155.1270	78.0671	138.1005	69.5539			1

Error Distribution Error Distribution (ppm)

NCBI BLAST search of SEYSVHSSLASK
(Parameters: blastp, nr protein database, expect=20000, no filter, PAM30)
Other BLAST web gateways

All matches to this query

Score Mr(calc): Delta Sequence

49.8 1381.6006 0.0052 SEYSVHSSLASK

39.4 1381.6006 0.0052 SEYSVHSSLASK

26.1 1381.6006 0.0052 SEYSVHSSLASK

6.1 1381.6126 -0.0068 VSSAEGAAKEEPK

6.1 1381.6126 -0.0068 VSSAEGAAKEEPK

5.5 1381.6159 -0.0101 CQTQPRSSPSGR

3.9 1381.6084 -0.0026 TAKQVASRVQK

3.8 1381.5993 0.0065 EQDSSSDELNKK

3.8 1381.5993 0.0065 EQDSSSDELNKK

3.0 1381.6067 -0.0009 DKEGNVLMDTEL

Score	Mr(calc):	Delta	Sequence
49.8	1381.6006	0.0052	SEYSVHSSLASK
39.4	1381.6006	0.0052	SEYSVHSSLASK
26.1	1381.6006	0.0052	SEYSVHSSLASK
6.1	1381.6126	-0.0068	VSSAEGAAKEEPK
6.1	1381.6126	-0.0068	VSSAEGAAKEEPK
5.5	1381.6159	-0.0101	CQTQPRSSPSGR
3.9	1381.6084	-0.0026	TAKQVASRVQK
3.8	1381.5993	0.0065	EQDSSSDELNKK
3.8	1381.5993	0.0065	EQDSSSDELNKK
3.0	1381.6067	-0.0009	DKEGNVLMDTEL

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