Mascot Search Results

Peptide View

MS/MS Fragmentation of THAVSVSETDDYAEIIDEEDTYTMPSTR
Found in ENSP00000342839, gene:PTK2 -- Focal adhesion kinase 1 (EC 2.7.10.2) (FADK 1) (pp125FAK) (Protein- tyrosine kinase

Match to Query 33241: 3254.354262 from(1085.792030,3+)
Title: Elution from: 777.777 to 777.777 period: 230208_dwb2_b1fc_5min_4g10_ip1.raw experiment: 1 cycles: 1 precIntensity: 30179.0 FinneganScanNumber: 7366
Data file 230208_DWB2_b1Fc_5min_conj.msm

Monoisotopic mass of neutral peptide Mr(calc): 3254.3486
Fixed modifications: Carbamidomethyl (C)
Variable modifications: 
Y12    : Phospho (Y)
Ions Score: 44  Expect: 0.21  
Matches (Bold Red): 24/268 fragment ions using 27 most intense peaks

#	b	b⁺⁺	b⁰	b⁰⁺⁺	Seq.	y	y⁺⁺	y*	y*⁺⁺	y⁰	y⁰⁺⁺	#
1	102.0550	51.5311	84.0444	42.5258	T							28
2	239.1139	120.0606	221.1033	111.0553	H	3154.3082	1577.6577	3137.2816	1569.1445	3136.2976	1568.6524	27
3	310.1510	155.5791	292.1404	146.5738	A	3017.2493	1509.1283	3000.2227	1500.6150	2999.2387	1500.1230	26
4	409.2194	205.1133	391.2088	196.1080	V	2946.2122	1473.6097	2929.1856	1465.0964	2928.2016	1464.6044	25
5	496.2514	248.6293	478.2409	239.6241	S	2847.1437	1424.0755	2830.1172	1415.5622	2829.1332	1415.0702	24
6	595.3198	298.1636	577.3093	289.1583	V	2760.1117	1380.5595	2743.0852	1372.0462	2742.1011	1371.5542	23
7	682.3519	341.6796	664.3413	332.6743	S	2661.0433	1331.0253	2644.0168	1322.5120	2643.0327	1322.0200	22
8	811.3944	406.2009	793.3839	397.1956	E	2574.0113	1287.5093	2556.9847	1278.9960	2556.0007	1278.5040	21
9	912.4421	456.7247	894.4316	447.7194	T	2444.9687	1222.9880	2427.9421	1214.4747	2426.9581	1213.9827	20
10	1027.4691	514.2382	1009.4585	505.2329	D	2343.9210	1172.4641	2326.8945	1163.9509	2325.9104	1163.4589	19
11	1142.4960	571.7516	1124.4854	562.7464	D	2228.8941	1114.9507	2211.8675	1106.4374	2210.8835	1105.9454	18
12	1385.5257	693.2665	1367.5151	684.2612	Y	2113.8671	1057.4372	2096.8406	1048.9239	2095.8566	1048.4319	17
13	1456.5628	728.7850	1438.5522	719.7797	A	1870.8375	935.9224	1853.8109	927.4091	1852.8269	926.9171	16
14	1585.6054	793.3063	1567.5948	784.3010	E	1799.8004	900.4038	1782.7738	891.8905	1781.7898	891.3985	15
15	1698.6894	849.8483	1680.6789	840.8431	I	1670.7578	835.8825	1653.7312	827.3692	1652.7472	826.8772	14
16	1811.7735	906.3904	1793.7629	897.3851	I	1557.6737	779.3405	1540.6472	770.8272	1539.6631	770.3352	13
17	1926.8004	963.9038	1908.7899	954.8986	D	1444.5897	722.7985	1427.5631	714.2852	1426.5791	713.7932	12
18	2055.8430	1028.4251	2037.8324	1019.4199	E	1329.5627	665.2850	1312.5362	656.7717	1311.5521	656.2797	11
19	2184.8856	1092.9464	2166.8750	1083.9412	E	1200.5201	600.7637	1183.4936	592.2504	1182.5096	591.7584	10
20	2299.9125	1150.4599	2281.9020	1141.4546	D	1071.4775	536.2424	1054.4510	527.7291	1053.4670	527.2371	9
21	2400.9602	1200.9837	2382.9497	1191.9785	T	956.4506	478.7289	939.4240	470.2157	938.4400	469.7237	8
22	2564.0235	1282.5154	2546.0130	1273.5101	Y	855.4029	428.2051	838.3764	419.6918	837.3923	419.1998	7
23	2665.0712	1333.0393	2647.0607	1324.0340	T	692.3396	346.6734	675.3130	338.1602	674.3290	337.6681	6
24	2796.1117	1398.5595	2778.1011	1389.5542	M	591.2919	296.1496	574.2654	287.6363	573.2813	287.1443	5
25	2893.1645	1447.0859	2875.1539	1438.0806	P	460.2514	230.6293	443.2249	222.1161	442.2409	221.6241	4
26	2980.1965	1490.6019	2962.1859	1481.5966	S	363.1987	182.1030	346.1721	173.5897	345.1881	173.0977	3
27	3081.2442	1541.1257	3063.2336	1532.1204	T	276.1666	138.5870	259.1401	130.0737	258.1561	129.5817	2
28					R	175.1190	88.0631	158.0924	79.5498			1

Error Distribution Error Distribution (ppm)

NCBI BLAST search of THAVSVSETDDYAEIIDEEDTYTMPSTR
(Parameters: blastp, nr protein database, expect=20000, no filter, PAM30)
Other BLAST web gateways

All matches to this query

Score Mr(calc): Delta Sequence

43.9 3254.3486 0.0057 THAVSVSETDDYAEIIDEEDTYTMPSTR

28.9 3254.3486 0.0057 THAVSVSETDDYAEIIDEEDTYTMPSTR

28.9 3254.3486 0.0057 THAVSVSETDDYAEIIDEEDTYTMPSTR

26.1 3254.3486 0.0057 THAVSVSETDDYAEIIDEEDTYTMPSTR

26.1 3254.3486 0.0057 THAVSVSETDDYAEIIDEEDTYTMPSTR

12.7 3254.3486 0.0057 THAVSVSETDDYAEIIDEEDTYTMPSTR

11.2 3254.3658 -0.0116 GSITTMLALASSQGDGLYKELADVGSEYK

10.5 3254.3658 -0.0116 GSITTMLALASSQGDGLYKELADVGSEYK

10.1 3254.3763 -0.0220 NSFSEIRIHPLSQASGGSSNIRQEPAPK

9.6 3254.3541 0.0002 STGLQADTEAKMKGLAKPTTSEVMLER

Score	Mr(calc):	Delta	Sequence
43.9	3254.3486	0.0057	THAVSVSETDDYAEIIDEEDTYTMPSTR
28.9	3254.3486	0.0057	THAVSVSETDDYAEIIDEEDTYTMPSTR
28.9	3254.3486	0.0057	THAVSVSETDDYAEIIDEEDTYTMPSTR
26.1	3254.3486	0.0057	THAVSVSETDDYAEIIDEEDTYTMPSTR
26.1	3254.3486	0.0057	THAVSVSETDDYAEIIDEEDTYTMPSTR
12.7	3254.3486	0.0057	THAVSVSETDDYAEIIDEEDTYTMPSTR
11.2	3254.3658	-0.0116	GSITTMLALASSQGDGLYKELADVGSEYK
10.5	3254.3658	-0.0116	GSITTMLALASSQGDGLYKELADVGSEYK
10.1	3254.3763	-0.0220	NSFSEIRIHPLSQASGGSSNIRQEPAPK
9.6	3254.3541	0.0002	STGLQADTEAKMKGLAKPTTSEVMLER

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