Mascot Search Results

Peptide View

MS/MS Fragmentation of EATQPEPIYAESTK
Found in ENSP00000330930, Tyrosine-protein kinase SgK223 (EC 2.7.10.2) (Sugen kinase 223). [Source:Uniprot/SWISSPROT;Acc:Q86

Match to Query 12085: 1650.728866 from(826.371709,2+)
Title: Elution from: 777.777 to 777.777 period: 181207_bidira_b1-b2d_10min_ip1.raw experiment: 1 cycles: 1 precIntensity: 54282.0 FinneganScanNumber: 3709
Data file 181207_BidirB_B2DIC_10min_conj.msm

Monoisotopic mass of neutral peptide Mr(calc): 1650.7269
Fixed modifications: Carbamidomethyl (C)
Variable modifications: 
Y9     : Phospho (Y)
K14    : Lysine-13C615N2 (K_8)
Ions Score: 54  Expect: 0.0053  
Matches (Bold Red): 36/148 fragment ions using 66 most intense peaks

#	b	b⁺⁺	b*	b*⁺⁺	b⁰	b⁰⁺⁺	Seq.	y	y⁺⁺	y*	y*⁺⁺	y⁰	y⁰⁺⁺	#
1	130.0499	65.5286			112.0393	56.5233	E							14
2	201.0870	101.0471			183.0764	92.0418	A	1522.6916	761.8494	1505.6651	753.3362	1504.6810	752.8442	13
3	302.1347	151.5710			284.1241	142.5657	T	1451.6545	726.3309	1434.6280	717.8176	1433.6439	717.3256	12
4	430.1932	215.6003	413.1667	207.0870	412.1827	206.5950	Q	1350.6068	675.8070	1333.5803	667.2938	1332.5963	666.8018	11
5	527.2460	264.1266	510.2194	255.6134	509.2354	255.1214	P	1222.5482	611.7778	1205.5217	603.2645	1204.5377	602.7725	10
6	656.2886	328.6479	639.2620	320.1347	638.2780	319.6426	E	1125.4955	563.2514	1108.4689	554.7381	1107.4849	554.2461	9
7	753.3413	377.1743	736.3148	368.6610	735.3308	368.1690	P	996.4529	498.7301	979.4263	490.2168	978.4423	489.7248	8
8	866.4254	433.7163	849.3989	425.2031	848.4148	424.7111	I	899.4001	450.2037	882.3736	441.6904	881.3896	441.1984	7
9	1109.4551	555.2312	1092.4285	546.7179	1091.4445	546.2259	Y	786.3161	393.6617	769.2895	385.1484	768.3055	384.6564	6
10	1180.4922	590.7497	1163.4656	582.2364	1162.4816	581.7444	A	543.2864	272.1468	526.2599	263.6336	525.2759	263.1416	5
11	1309.5348	655.2710	1292.5082	646.7577	1291.5242	646.2657	E	472.2493	236.6283	455.2228	228.1150	454.2387	227.6230	4
12	1396.5668	698.7870	1379.5402	690.2738	1378.5562	689.7818	S	343.2067	172.1070	326.1802	163.5937	325.1962	163.1017	3
13	1497.6145	749.3109	1480.5879	740.7976	1479.6039	740.3056	T	256.1747	128.5910	239.1481	120.0777	238.1641	119.5857	2
14							K	155.1270	78.0671	138.1005	69.5539			1

Error Distribution Error Distribution (ppm)

NCBI BLAST search of EATQPEPIYAESTK
(Parameters: blastp, nr protein database, expect=20000, no filter, PAM30)
Other BLAST web gateways

All matches to this query

Score Mr(calc): Delta Sequence

54.4 1650.7269 0.0020 EATQPEPIYAESTK

17.8 1650.7421 -0.0133 SGVDADSSYFKIFK

12.9 1650.7347 -0.0058 SGRDPLAILSEISK

10.3 1650.7202 0.0087 RGSEMDNNCSPTRK

9.7 1650.7528 -0.0239 ITGRAHWGSHHAGGK

9.1 1650.7554 -0.0266 GDQQDKLLESLNCK

8.7 1650.6959 0.0330 FKEKENSECAFR

8.4 1650.7452 -0.0163 NDAQKQLSEEQDAR

8.0 1650.7480 -0.0192 EDSNLTLQEKKEK

7.7 1650.7475 -0.0186 ATQQSLGEQSNPRR

Score	Mr(calc):	Delta	Sequence
54.4	1650.7269	0.0020	EATQPEPIYAESTK
17.8	1650.7421	-0.0133	SGVDADSSYFKIFK
12.9	1650.7347	-0.0058	SGRDPLAILSEISK
10.3	1650.7202	0.0087	RGSEMDNNCSPTRK
9.7	1650.7528	-0.0239	ITGRAHWGSHHAGGK
9.1	1650.7554	-0.0266	GDQQDKLLESLNCK
8.7	1650.6959	0.0330	FKEKENSECAFR
8.4	1650.7452	-0.0163	NDAQKQLSEEQDAR
8.0	1650.7480	-0.0192	EDSNLTLQEKKEK
7.7	1650.7475	-0.0186	ATQQSLGEQSNPRR

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