Mascot Search Results

Peptide View

MS/MS Fragmentation of GYYSPYSVSGSGSTAGSR
Found in ENSP00000323856, gene:PLEC1 -- Plectin-1 (PLTN) (PCN) (Hemidesmosomal protein 1) (HD1) (Plectin-11). [Source:Unipr

Match to Query 20141: 1861.759784 from(931.887168,2+)
Title: Elution from: 777.777 to 777.777 period: 230208_dwb2_b1fc_5min_4g10_ip1.raw experiment: 1 cycles: 1 precIntensity: 23867.0 FinneganScanNumber: 3687
Data file 230208_DWB2_b1Fc_5min_conj.msm

Monoisotopic mass of neutral peptide Mr(calc): 1861.7519
Fixed modifications: Carbamidomethyl (C)
Variable modifications: 
Y6     : Phospho (Y)
Ions Score: 93  Expect: 6.8e-07  
Matches (Bold Red): 35/162 fragment ions using 61 most intense peaks

#	b	b⁺⁺	b⁰	b⁰⁺⁺	Seq.	y	y⁺⁺	y*	y*⁺⁺	y⁰	y⁰⁺⁺	#
1	58.0287	29.5180			G							18
2	221.0921	111.0497			Y	1805.7378	903.3725	1788.7112	894.8593	1787.7272	894.3672	17
3	384.1554	192.5813			Y	1642.6745	821.8409	1625.6479	813.3276	1624.6639	812.8356	16
4	471.1874	236.0974	453.1769	227.0921	S	1479.6111	740.3092	1462.5846	731.7959	1461.6006	731.3039	15
5	568.2402	284.6237	550.2296	275.6184	P	1392.5791	696.7932	1375.5525	688.2799	1374.5685	687.7879	14
6	811.2698	406.1386	793.2593	397.1333	Y	1295.5263	648.2668	1278.4998	639.7535	1277.5158	639.2615	13
7	898.3019	449.6546	880.2913	440.6493	S	1052.4967	526.7520	1035.4701	518.2387	1034.4861	517.7467	12
8	997.3703	499.1888	979.3597	490.1835	V	965.4647	483.2360	948.4381	474.7227	947.4541	474.2307	11
9	1084.4023	542.7048	1066.3917	533.6995	S	866.3962	433.7018	849.3697	425.1885	848.3857	424.6965	10
10	1141.4238	571.2155	1123.4132	562.2102	G	779.3642	390.1857	762.3377	381.6725	761.3536	381.1805	9
11	1228.4558	614.7315	1210.4452	605.7263	S	722.3428	361.6750	705.3162	353.1617	704.3322	352.6697	8
12	1285.4773	643.2423	1267.4667	634.2370	G	635.3107	318.1590	618.2842	309.6457	617.3002	309.1537	7
13	1372.5093	686.7583	1354.4987	677.7530	S	578.2893	289.6483	561.2627	281.1350	560.2787	280.6430	6
14	1473.5570	737.2821	1455.5464	728.2768	T	491.2572	246.1323	474.2307	237.6190	473.2467	237.1270	5
15	1544.5941	772.8007	1526.5835	763.7954	A	390.2096	195.6084	373.1830	187.0951	372.1990	186.6031	4
16	1601.6155	801.3114	1583.6050	792.3061	G	319.1724	160.0899	302.1459	151.5766	301.1619	151.0846	3
17	1688.6476	844.8274	1670.6370	835.8221	S	262.1510	131.5791	245.1244	123.0659	244.1404	122.5738	2
18					R	175.1190	88.0631	158.0924	79.5498			1

Error Distribution Error Distribution (ppm)

NCBI BLAST search of GYYSPYSVSGSGSTAGSR
(Parameters: blastp, nr protein database, expect=20000, no filter, PAM30)
Other BLAST web gateways

All matches to this query

Score Mr(calc): Delta Sequence

93.3 1861.7519 0.0079 GYYSPYSVSGSGSTAGSR

44.6 1861.7519 0.0079 GYYSPYSVSGSGSTAGSR

33.3 1861.7519 0.0079 GYYSPYSVSGSGSTAGSR

32.0 1861.7519 0.0079 GYYSPYSVSGSGSTAGSR

25.6 1861.7519 0.0079 GYYSPYSVSGSGSTAGSR

16.6 1861.7519 0.0079 GYYSPYSVSGSGSTAGSR

10.6 1861.7519 0.0079 GYYSPYSVSGSGSTAGSR

7.8 1861.7287 0.0310 SQSRSYSSADSYSSTR

7.2 1861.7888 -0.0290 RQSKDSAWDPSQTMK

7.2 1861.7888 -0.0290 RQSKDSAWDPSQTMK

Score	Mr(calc):	Delta	Sequence
93.3	1861.7519	0.0079	GYYSPYSVSGSGSTAGSR
44.6	1861.7519	0.0079	GYYSPYSVSGSGSTAGSR
33.3	1861.7519	0.0079	GYYSPYSVSGSGSTAGSR
32.0	1861.7519	0.0079	GYYSPYSVSGSGSTAGSR
25.6	1861.7519	0.0079	GYYSPYSVSGSGSTAGSR
16.6	1861.7519	0.0079	GYYSPYSVSGSGSTAGSR
10.6	1861.7519	0.0079	GYYSPYSVSGSGSTAGSR
7.8	1861.7287	0.0310	SQSRSYSSADSYSSTR
7.2	1861.7888	-0.0290	RQSKDSAWDPSQTMK
7.2	1861.7888	-0.0290	RQSKDSAWDPSQTMK

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