Mascot Search Results

Peptide View

MS/MS Fragmentation of SQSEYKPDTPQSGLEYSGIQELEDR
Found in ENSP00000359544, gene:PKN2 -- Serine/threonine-protein kinase N2 (EC 2.7.11.13) (Protein kinase C- like 2) (Protei

Match to Query 32448: 2935.285428 from(979.435752,3+)
Title: Elution from: 777.777 to 777.777 period: 230208_dwb2_b1fc_5min_4g10_ip1.raw experiment: 1 cycles: 1 precIntensity: 18414.0 FinneganScanNumber: 7166
Data file 230208_DWB2_b1Fc_5min_conj.msm

Monoisotopic mass of neutral peptide Mr(calc): 2935.2760
Fixed modifications: Carbamidomethyl (C)
Variable modifications: 
Y16    : Phospho (Y)
Ions Score: 63  Expect: 0.0023  
Matches (Bold Red): 34/284 fragment ions using 37 most intense peaks

#	b	b⁺⁺	b*	b*⁺⁺	b⁰	b⁰⁺⁺	Seq.	y	y⁺⁺	y*	y*⁺⁺	y⁰	y⁰⁺⁺	#
1	88.0393	44.5233			70.0287	35.5180	S							25
2	216.0979	108.5526	199.0713	100.0393	198.0873	99.5473	Q	2849.2513	1425.1293	2832.2247	1416.6160	2831.2407	1416.1240	24
3	303.1299	152.0686	286.1034	143.5553	285.1194	143.0633	S	2721.1927	1361.1000	2704.1661	1352.5867	2703.1821	1352.0947	23
4	432.1725	216.5899	415.1460	208.0766	414.1619	207.5846	E	2634.1607	1317.5840	2617.1341	1309.0707	2616.1501	1308.5787	22
5	595.2358	298.1216	578.2093	289.6083	577.2253	289.1163	Y	2505.1181	1253.0627	2488.0915	1244.5494	2487.1075	1244.0574	21
6	723.3308	362.1690	706.3042	353.6558	705.3202	353.1638	K	2342.0547	1171.5310	2325.0282	1163.0177	2324.0442	1162.5257	20
7	820.3836	410.6954	803.3570	402.1821	802.3730	401.6901	P	2213.9598	1107.4835	2196.9332	1098.9703	2195.9492	1098.4782	19
8	935.4105	468.2089	918.3839	459.6956	917.3999	459.2036	D	2116.9070	1058.9571	2099.8805	1050.4439	2098.8964	1049.9519	18
9	1036.4582	518.7327	1019.4316	510.2195	1018.4476	509.7274	T	2001.8801	1001.4437	1984.8535	992.9304	1983.8695	992.4384	17
10	1133.5109	567.2591	1116.4844	558.7458	1115.5004	558.2538	P	1900.8324	950.9198	1883.8058	942.4066	1882.8218	941.9146	16
11	1261.5695	631.2884	1244.5430	622.7751	1243.5590	622.2831	Q	1803.7796	902.3935	1786.7531	893.8802	1785.7691	893.3882	15
12	1348.6015	674.8044	1331.5750	666.2911	1330.5910	665.7991	S	1675.7211	838.3642	1658.6945	829.8509	1657.7105	829.3589	14
13	1405.6230	703.3151	1388.5965	694.8019	1387.6124	694.3099	G	1588.6890	794.8482	1571.6625	786.3349	1570.6785	785.8429	13
14	1518.7071	759.8572	1501.6805	751.3439	1500.6965	750.8519	L	1531.6676	766.3374	1514.6410	757.8241	1513.6570	757.3321	12
15	1647.7497	824.3785	1630.7231	815.8652	1629.7391	815.3732	E	1418.5835	709.7954	1401.5570	701.2821	1400.5729	700.7901	11
16	1890.7793	945.8933	1873.7528	937.3800	1872.7687	936.8880	Y	1289.5409	645.2741	1272.5144	636.7608	1271.5304	636.2688	10
17	1977.8113	989.4093	1960.7848	980.8960	1959.8008	980.4040	S	1046.5113	523.7593	1029.4847	515.2460	1028.5007	514.7540	9
18	2034.8328	1017.9200	2017.8063	1009.4068	2016.8222	1008.9148	G	959.4792	480.2433	942.4527	471.7300	941.4687	471.2380	8
19	2147.9169	1074.4621	2130.8903	1065.9488	2129.9063	1065.4568	I	902.4578	451.7325	885.4312	443.2192	884.4472	442.7272	7
20	2275.9754	1138.4914	2258.9489	1129.9781	2257.9649	1129.4861	Q	789.3737	395.1905	772.3472	386.6772	771.3631	386.1852	6
21	2405.0180	1203.0127	2387.9915	1194.4994	2387.0075	1194.0074	E	661.3151	331.1612	644.2886	322.6479	643.3046	322.1559	5
22	2518.1021	1259.5547	2501.0755	1251.0414	2500.0915	1250.5494	L	532.2725	266.6399	515.2460	258.1266	514.2620	257.6346	4
23	2647.1447	1324.0760	2630.1181	1315.5627	2629.1341	1315.0707	E	419.1885	210.0979	402.1619	201.5846	401.1779	201.0926	3
24	2762.1716	1381.5894	2745.1451	1373.0762	2744.1611	1372.5842	D	290.1459	145.5766	273.1193	137.0633	272.1353	136.5713	2
25							R	175.1190	88.0631	158.0924	79.5498			1

Error Distribution Error Distribution (ppm)

NCBI BLAST search of SQSEYKPDTPQSGLEYSGIQELEDR
(Parameters: blastp, nr protein database, expect=20000, no filter, PAM30)
Other BLAST web gateways

All matches to this query

Score Mr(calc): Delta Sequence

63.3 2935.2760 0.0094 SQSEYKPDTPQSGLEYSGIQELEDR

20.0 2935.2760 0.0094 SQSEYKPDTPQSGLEYSGIQELEDR

14.8 2935.2760 0.0094 SQSEYKPDTPQSGLEYSGIQELEDR

14.8 2935.2760 0.0094 SQSEYKPDTPQSGLEYSGIQELEDR

14.8 2935.2760 0.0094 SQSEYKPDTPQSGLEYSGIQELEDR

14.8 2935.2760 0.0094 SQSEYKPDTPQSGLEYSGIQELEDR

13.7 2935.3400 -0.0545 KERPHMLNLSGTDSSGVLRPRQNR

13.7 2935.3400 -0.0545 KERPHMLNLSGTDSSGVLRPRQNR

13.7 2935.3400 -0.0545 KERPHMLNLSGTDSSGVLRPRQNR

13.7 2935.3400 -0.0545 KERPHMLNLSGTDSSGVLRPRQNR

Score	Mr(calc):	Delta	Sequence
63.3	2935.2760	0.0094	SQSEYKPDTPQSGLEYSGIQELEDR
20.0	2935.2760	0.0094	SQSEYKPDTPQSGLEYSGIQELEDR
14.8	2935.2760	0.0094	SQSEYKPDTPQSGLEYSGIQELEDR
14.8	2935.2760	0.0094	SQSEYKPDTPQSGLEYSGIQELEDR
14.8	2935.2760	0.0094	SQSEYKPDTPQSGLEYSGIQELEDR
14.8	2935.2760	0.0094	SQSEYKPDTPQSGLEYSGIQELEDR
13.7	2935.3400	-0.0545	KERPHMLNLSGTDSSGVLRPRQNR
13.7	2935.3400	-0.0545	KERPHMLNLSGTDSSGVLRPRQNR
13.7	2935.3400	-0.0545	KERPHMLNLSGTDSSGVLRPRQNR
13.7	2935.3400	-0.0545	KERPHMLNLSGTDSSGVLRPRQNR

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