Mascot Search Results

Peptide View

MS/MS Fragmentation of NKDQGTYEDYVEGLR
Found in ENSP00000293422, gene:MYL6 -- Myosin light polypeptide 6 (Smooth muscle and nonmuscle myosin light chain alkali 6)

Match to Query 20198: 1865.794132 from(933.904342,2+)
Title: Elution from: 777.777 to 777.777 period: 230208_b2dw_b1fc_5min_4g10_ip2.raw experiment: 1 cycles: 1 precIntensity: 32646.0 FinneganScanNumber: 6353
Data file 230208_DWB2_b1Fc_5min_conj.msm

Monoisotopic mass of neutral peptide Mr(calc): 1865.7832
Fixed modifications: Carbamidomethyl (C)
Variable modifications: 
Y7     : Phospho (Y)
Ions Score: 51  Expect: 0.013  
Matches (Bold Red): 27/158 fragment ions using 36 most intense peaks

#	b	b⁺⁺	b*	b*⁺⁺	b⁰	b⁰⁺⁺	Seq.	y	y⁺⁺	y*	y*⁺⁺	y⁰	y⁰⁺⁺	#
1	115.0502	58.0287	98.0237	49.5155			N							15
2	243.1452	122.0762	226.1186	113.5629			K	1752.7476	876.8774	1735.7211	868.3642	1734.7370	867.8722	14
3	358.1721	179.5897	341.1456	171.0764	340.1615	170.5844	D	1624.6526	812.8300	1607.6261	804.3167	1606.6421	803.8247	13
4	486.2307	243.6190	469.2041	235.1057	468.2201	234.6137	Q	1509.6257	755.3165	1492.5992	746.8032	1491.6151	746.3112	12
5	543.2521	272.1297	526.2256	263.6164	525.2416	263.1244	G	1381.5671	691.2872	1364.5406	682.7739	1363.5566	682.2819	11
6	644.2998	322.6536	627.2733	314.1403	626.2893	313.6483	T	1324.5457	662.7765	1307.5191	654.2632	1306.5351	653.7712	10
7	887.3295	444.1684	870.3029	435.6551	869.3189	435.1631	Y	1223.4980	612.2526	1206.4714	603.7394	1205.4874	603.2473	9
8	1016.3721	508.6897	999.3455	500.1764	998.3615	499.6844	E	980.4683	490.7378	963.4418	482.2245	962.4578	481.7325	8
9	1131.3990	566.2031	1114.3725	557.6899	1113.3884	557.1979	D	851.4257	426.2165	834.3992	417.7032	833.4152	417.2112	7
10	1294.4623	647.7348	1277.4358	639.2215	1276.4518	638.7295	Y	736.3988	368.7030	719.3723	360.1898	718.3882	359.6978	6
11	1393.5308	697.2690	1376.5042	688.7557	1375.5202	688.2637	V	573.3355	287.1714	556.3089	278.6581	555.3249	278.1661	5
12	1522.5733	761.7903	1505.5468	753.2770	1504.5628	752.7850	E	474.2671	237.6372	457.2405	229.1239	456.2565	228.6319	4
13	1579.5948	790.3010	1562.5683	781.7878	1561.5842	781.2958	G	345.2245	173.1159	328.1979	164.6026			3
14	1692.6789	846.8431	1675.6523	838.3298	1674.6683	837.8378	L	288.2030	144.6051	271.1765	136.0919			2
15							R	175.1190	88.0631	158.0924	79.5498			1

Error Distribution Error Distribution (ppm)

NCBI BLAST search of NKDQGTYEDYVEGLR
(Parameters: blastp, nr protein database, expect=20000, no filter, PAM30)
Other BLAST web gateways

All matches to this query

Score Mr(calc): Delta Sequence

51.4 1865.7832 0.0109 NKDQGTYEDYVEGLR

26.5 1865.7832 0.0109 NKDQGTYEDYVEGLR

18.9 1865.7832 0.0109 NKDQGTYEDYVEGLR

12.4 1865.8225 -0.0283 QLAQKYNCDKMICR

11.8 1865.7767 0.0174 QNHIYQQNHMVLSR

10.7 1865.8303 -0.0361 FIPCSPFSDYVYKR

6.7 1865.7640 0.0302 YNDNFWKEFGTNIK

6.6 1865.7898 0.0043 MEPKASCPAAAPLMER

6.6 1865.8076 -0.0135 SSTVGKMLSNSIACYR

4.1 1865.8303 -0.0361 FIPCSPFSDYVYKR

Score	Mr(calc):	Delta	Sequence
51.4	1865.7832	0.0109	NKDQGTYEDYVEGLR
26.5	1865.7832	0.0109	NKDQGTYEDYVEGLR
18.9	1865.7832	0.0109	NKDQGTYEDYVEGLR
12.4	1865.8225	-0.0283	QLAQKYNCDKMICR
11.8	1865.7767	0.0174	QNHIYQQNHMVLSR
10.7	1865.8303	-0.0361	FIPCSPFSDYVYKR
6.7	1865.7640	0.0302	YNDNFWKEFGTNIK
6.6	1865.7898	0.0043	MEPKASCPAAAPLMER
6.6	1865.8076	-0.0135	SSTVGKMLSNSIACYR
4.1	1865.8303	-0.0361	FIPCSPFSDYVYKR

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