Mascot Search Results

Peptide View

MS/MS Fragmentation of NEEYFMFPEPK
Found in ENSP00000298048, gene:MELK -- Maternal embryonic leucine zipper kinase (EC 2.7.11.1) (hMELK) (Protein kinase PK38)

Match to Query 17401: 1509.594430 from(755.804491,2+)
Title: Elution from: 777.777 to 777.777 period: 100308_b2dw_b1fc_5minptyr100_ip2.raw experiment: 1 cycles: 1 precIntensity: 6780.0 FinneganScanNumber: 7912
Data file 100308_DWB2_b1Fc_5min_conj.msm

Monoisotopic mass of neutral peptide Mr(calc): 1509.5887
Fixed modifications: Carbamidomethyl (C)
Variable modifications: 
Y4     : Phospho (Y)
Ions Score: 51  Expect: 0.0036  
Matches (Bold Red): 14/114 fragment ions using 14 most intense peaks

#	b	b⁺⁺	b*	b*⁺⁺	b⁰	b⁰⁺⁺	Seq.	y	y⁺⁺	y*	y*⁺⁺	y⁰	y⁰⁺⁺	#
1	115.0502	58.0287	98.0237	49.5155			N							11
2	244.0928	122.5500	227.0662	114.0368	226.0822	113.5448	E	1396.5530	698.7802	1379.5265	690.2669	1378.5425	689.7749	10
3	373.1354	187.0713	356.1088	178.5581	355.1248	178.0660	E	1267.5105	634.2589	1250.4839	625.7456	1249.4999	625.2536	9
4	616.1650	308.5862	599.1385	300.0729	598.1545	299.5809	Y	1138.4679	569.7376	1121.4413	561.2243	1120.4573	560.7323	8
5	763.2335	382.1204	746.2069	373.6071	745.2229	373.1151	F	895.4382	448.2227	878.4117	439.7095	877.4276	439.2175	7
6	894.2739	447.6406	877.2474	439.1273	876.2634	438.6353	M	748.3698	374.6885	731.3433	366.1753	730.3592	365.6833	6
7	1041.3423	521.1748	1024.3158	512.6615	1023.3318	512.1695	F	617.3293	309.1683	600.3028	300.6550	599.3188	300.1630	5
8	1138.3951	569.7012	1121.3686	561.1879	1120.3845	560.6959	P	470.2609	235.6341	453.2344	227.1208	452.2503	226.6288	4
9	1267.4377	634.2225	1250.4111	625.7092	1249.4271	625.2172	E	373.2082	187.1077	356.1816	178.5944	355.1976	178.1024	3
10	1364.4905	682.7489	1347.4639	674.2356	1346.4799	673.7436	P	244.1656	122.5864	227.1390	114.0731			2
11							K	147.1128	74.0600	130.0863	65.5468			1

Error Distribution Error Distribution (ppm)

NCBI BLAST search of NEEYFMFPEPK
(Parameters: blastp, nr protein database, expect=20000, no filter, PAM30)
Other BLAST web gateways

All matches to this query

Score Mr(calc): Delta Sequence

50.7 1509.5887 0.0058 NEEYFMFPEPK

11.8 1509.6012 -0.0067 YEIMNFKEMGK

9.7 1509.5921 0.0024 DKEPLTAYNYK

8.8 1509.6115 -0.0171 YENEERPEVQK

5.8 1509.6078 -0.0134 YEENMWPGSRK

5.8 1509.6012 -0.0067 YEIMNFKEMGK

5.5 1509.5977 -0.0033 YLENYYDLKK

5.5 1509.5977 -0.0033 YLENYYDLKK

3.3 1509.6049 -0.0105 YEIKMTKMYK

3.3 1509.6049 -0.0105 YEIKMTKMYK

Score	Mr(calc):	Delta	Sequence
50.7	1509.5887	0.0058	NEEYFMFPEPK
11.8	1509.6012	-0.0067	YEIMNFKEMGK
9.7	1509.5921	0.0024	DKEPLTAYNYK
8.8	1509.6115	-0.0171	YENEERPEVQK
5.8	1509.6078	-0.0134	YEENMWPGSRK
5.8	1509.6012	-0.0067	YEIMNFKEMGK
5.5	1509.5977	-0.0033	YLENYYDLKK
5.5	1509.5977	-0.0033	YLENYYDLKK
3.3	1509.6049	-0.0105	YEIKMTKMYK
3.3	1509.6049	-0.0105	YEIKMTKMYK

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