Mascot Search Results

Peptide View

MS/MS Fragmentation of IADPEHDHTGFLTEYVATR
Found in ENSP00000327293, gene:MAPK3 -- Mitogen-activated protein kinase 3 (EC 2.7.11.24) (Extracellular signal-regulated k

Match to Query 7101: 2261.012406 from(754.678078,3+)
Title: Elution from: 777.777 to 777.777 period: 091207_bidira_10min_2e6_4g10.raw experiment: 1 cycles: 1 precIntensity: 74076.0 FinneganScanNumber: 4233
Data file 091207_BidirA_b1_10min_conj.msm

Monoisotopic mass of neutral peptide Mr(calc): 2261.0029
Fixed modifications: Carbamidomethyl (C)
Variable modifications: 
Y15    : Phospho (Y)
R19    : Arginine-13C615N4 (R_10)
Ions Score: 72  Expect: 0.00021  
Matches (Bold Red): 42/174 fragment ions using 64 most intense peaks

#	b	b⁺⁺	b⁰	b⁰⁺⁺	Seq.	y	y⁺⁺	y*	y*⁺⁺	y⁰	y⁰⁺⁺	#
1	114.0913	57.5493			I							19
2	185.1284	93.0679			A	2148.9261	1074.9667	2131.8996	1066.4534	2130.9156	1065.9614	18
3	300.1554	150.5813	282.1448	141.5760	D	2077.8890	1039.4481	2060.8625	1030.9349	2059.8785	1030.4429	17
4	397.2081	199.1077	379.1976	190.1024	P	1962.8621	981.9347	1945.8355	973.4214	1944.8515	972.9294	16
5	526.2507	263.6290	508.2402	254.6237	E	1865.8093	933.4083	1848.7828	924.8950	1847.7988	924.4030	15
6	663.3096	332.1585	645.2991	323.1532	H	1736.7667	868.8870	1719.7402	860.3737	1718.7562	859.8817	14
7	778.3366	389.6719	760.3260	380.6666	D	1599.7078	800.3575	1582.6813	791.8443	1581.6973	791.3523	13
8	915.3955	458.2014	897.3849	449.1961	H	1484.6809	742.8441	1467.6543	734.3308	1466.6703	733.8388	12
9	1016.4432	508.7252	998.4326	499.7199	T	1347.6220	674.3146	1330.5954	665.8013	1329.6114	665.3093	11
10	1073.4646	537.2360	1055.4541	528.2307	G	1246.5743	623.7908	1229.5477	615.2775	1228.5637	614.7855	10
11	1220.5330	610.7702	1202.5225	601.7649	F	1189.5528	595.2801	1172.5263	586.7668	1171.5423	586.2748	9
12	1333.6171	667.3122	1315.6065	658.3069	L	1042.4844	521.7458	1025.4579	513.2326	1024.4739	512.7406	8
13	1434.6648	717.8360	1416.6542	708.8307	T	929.4004	465.2038	912.3738	456.6905	911.3898	456.1985	7
14	1563.7074	782.3573	1545.6968	773.3520	E	828.3527	414.6800	811.3261	406.1667	810.3421	405.6747	6
15	1806.7370	903.8722	1788.7265	894.8669	Y	699.3101	350.1587	682.2835	341.6454	681.2995	341.1534	5
16	1905.8054	953.4064	1887.7949	944.4011	V	456.2804	228.6439	439.2539	220.1306	438.2699	219.6386	4
17	1976.8425	988.9249	1958.8320	979.9196	A	357.2120	179.1097	340.1855	170.5964	339.2015	170.1044	3
18	2077.8902	1039.4488	2059.8797	1030.4435	T	286.1749	143.5911	269.1484	135.0778	268.1644	134.5858	2
19					R	185.1272	93.0673	168.1007	84.5540			1

Error Distribution Error Distribution (ppm)

NCBI BLAST search of IADPEHDHTGFLTEYVATR
(Parameters: blastp, nr protein database, expect=20000, no filter, PAM30)
Other BLAST web gateways

All matches to this query

Score Mr(calc): Delta Sequence

71.7 2261.0029 0.0095 IADPEHDHTGFLTEYVATR

8.3 2261.0475 -0.0351 WQQEVAETVFDTINAETDR

8.2 2261.0029 0.0095 IADPEHDHTGFLTEYVATR

6.4 2260.9775 0.0349 GDTTSPSRPPGGPIITVHPK

5.8 2261.0345 -0.0220 NGKYDMSPIHQDKMNFIK

5.7 2261.0509 -0.0385 HGFNLVTSDIHNKTRLTK

5.4 2260.9775 0.0349 GDTTSPSRPPGGPIITVHPK

5.3 2260.9703 0.0421 ALNNVSLSSGDQRSRVAYR

5.3 2260.9703 0.0421 ALNNVSLSSGDQRSRVAYR

5.3 2260.9703 0.0421 ALNNVSLSSGDQRSRVAYR

Score	Mr(calc):	Delta	Sequence
71.7	2261.0029	0.0095	IADPEHDHTGFLTEYVATR
8.3	2261.0475	-0.0351	WQQEVAETVFDTINAETDR
8.2	2261.0029	0.0095	IADPEHDHTGFLTEYVATR
6.4	2260.9775	0.0349	GDTTSPSRPPGGPIITVHPK
5.8	2261.0345	-0.0220	NGKYDMSPIHQDKMNFIK
5.7	2261.0509	-0.0385	HGFNLVTSDIHNKTRLTK
5.4	2260.9775	0.0349	GDTTSPSRPPGGPIITVHPK
5.3	2260.9703	0.0421	ALNNVSLSSGDQRSRVAYR
5.3	2260.9703	0.0421	ALNNVSLSSGDQRSRVAYR
5.3	2260.9703	0.0421	ALNNVSLSSGDQRSRVAYR

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