Mascot Search Results

Peptide View

MS/MS Fragmentation of IADPEHDHTGFLTEYVATR
Found in ENSP00000263025, gene:MAPK3 -- Mitogen-activated protein kinase 3 (EC 2.7.11.24) (Extracellular signal-regulated k

Match to Query 26193: 2251.004325 from(751.342051,3+)
Title: Elution from: 777.777 to 777.777 period: 230208_dwb2_b1fc_5min_4g10_ip1.raw experiment: 1 cycles: 1 precIntensity: 89136.0 FinneganScanNumber: 5780
Data file 230208_DWB2_b1Fc_5min_conj.msm

Monoisotopic mass of neutral peptide Mr(calc): 2250.9946
Fixed modifications: Carbamidomethyl (C)
Variable modifications: 
Y15    : Phospho (Y)
Ions Score: 65  Expect: 0.0011  
Matches (Bold Red): 34/174 fragment ions using 43 most intense peaks

#	b	b⁺⁺	b⁰	b⁰⁺⁺	Seq.	y	y⁺⁺	y*	y*⁺⁺	y⁰	y⁰⁺⁺	#
1	114.0913	57.5493			I							19
2	185.1284	93.0679			A	2138.9178	1069.9626	2121.8913	1061.4493	2120.9073	1060.9573	18
3	300.1554	150.5813	282.1448	141.5760	D	2067.8807	1034.4440	2050.8542	1025.9307	2049.8702	1025.4387	17
4	397.2081	199.1077	379.1976	190.1024	P	1952.8538	976.9305	1935.8272	968.4173	1934.8432	967.9253	16
5	526.2507	263.6290	508.2402	254.6237	E	1855.8010	928.4042	1838.7745	919.8909	1837.7905	919.3989	15
6	663.3096	332.1585	645.2991	323.1532	H	1726.7584	863.8829	1709.7319	855.3696	1708.7479	854.8776	14
7	778.3366	389.6719	760.3260	380.6666	D	1589.6995	795.3534	1572.6730	786.8401	1571.6890	786.3481	13
8	915.3955	458.2014	897.3849	449.1961	H	1474.6726	737.8399	1457.6460	729.3267	1456.6620	728.8347	12
9	1016.4432	508.7252	998.4326	499.7199	T	1337.6137	669.3105	1320.5871	660.7972	1319.6031	660.3052	11
10	1073.4646	537.2360	1055.4541	528.2307	G	1236.5660	618.7866	1219.5395	610.2734	1218.5554	609.7814	10
11	1220.5330	610.7702	1202.5225	601.7649	F	1179.5445	590.2759	1162.5180	581.7626	1161.5340	581.2706	9
12	1333.6171	667.3122	1315.6065	658.3069	L	1032.4761	516.7417	1015.4496	508.2284	1014.4656	507.7364	8
13	1434.6648	717.8360	1416.6542	708.8307	T	919.3921	460.1997	902.3655	451.6864	901.3815	451.1944	7
14	1563.7074	782.3573	1545.6968	773.3520	E	818.3444	409.6758	801.3178	401.1626	800.3338	400.6706	6
15	1806.7370	903.8722	1788.7265	894.8669	Y	689.3018	345.1545	672.2753	336.6413	671.2912	336.1493	5
16	1905.8054	953.4064	1887.7949	944.4011	V	446.2722	223.6397	429.2456	215.1264	428.2616	214.6344	4
17	1976.8425	988.9249	1958.8320	979.9196	A	347.2037	174.1055	330.1772	165.5922	329.1932	165.1002	3
18	2077.8902	1039.4488	2059.8797	1030.4435	T	276.1666	138.5870	259.1401	130.0737	258.1561	129.5817	2
19					R	175.1190	88.0631	158.0924	79.5498			1

Error Distribution Error Distribution (ppm)

NCBI BLAST search of IADPEHDHTGFLTEYVATR
(Parameters: blastp, nr protein database, expect=20000, no filter, PAM30)
Other BLAST web gateways

All matches to this query

Score Mr(calc): Delta Sequence

64.6 2250.9946 0.0097 IADPEHDHTGFLTEYVATR

15.3 2250.9946 0.0097 IADPEHDHTGFLTEYVATR

9.9 2250.9672 0.0371 SKELVSSSSSGSDPDSEVDKK

9.9 2250.9672 0.0371 SKELVSSSSSGSDPDSEVDKK

9.8 2251.0360 -0.0317 IQGPQEYMVRSHNAGGSHPR

9.8 2250.9754 0.0289 FGLTPEQFGENLRDSYQR

9.7 2251.0160 -0.0117 EFLQTESPESTELQSRLR

9.6 2251.0044 -0.0001 RAKLVACDGNEIDTMFMDR

8.1 2250.9754 0.0289 FGLTPEQFGENLRDSYQR

7.7 2250.9951 0.0092 KGKGNSSMDSAAGCSGTPPICR

Score	Mr(calc):	Delta	Sequence
64.6	2250.9946	0.0097	IADPEHDHTGFLTEYVATR
15.3	2250.9946	0.0097	IADPEHDHTGFLTEYVATR
9.9	2250.9672	0.0371	SKELVSSSSSGSDPDSEVDKK
9.9	2250.9672	0.0371	SKELVSSSSSGSDPDSEVDKK
9.8	2251.0360	-0.0317	IQGPQEYMVRSHNAGGSHPR
9.8	2250.9754	0.0289	FGLTPEQFGENLRDSYQR
9.7	2251.0160	-0.0117	EFLQTESPESTELQSRLR
9.6	2251.0044	-0.0001	RAKLVACDGNEIDTMFMDR
8.1	2250.9754	0.0289	FGLTPEQFGENLRDSYQR
7.7	2250.9951	0.0092	KGKGNSSMDSAAGCSGTPPICR

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