Mascot Search Results

Peptide View

MS/MS Fragmentation of QADSEMTGYVVTR
Found in ENSP00000215659, gene:MAPK12 -- Mitogen-activated protein kinase 12 (EC 2.7.11.24) (Extracellular signal-regulated

Match to Query 18056: 1545.649444 from(773.831998,2+)
Title: Elution from: 777.777 to 777.777 period: 100308_b2dw_b1fc_5min_4g10_ip2.raw experiment: 1 cycles: 1 precIntensity: 16077.0 FinneganScanNumber: 5693
Data file 100308_DWB2_b1Fc_5min_conj.msm

Monoisotopic mass of neutral peptide Mr(calc): 1545.6410
Fixed modifications: Carbamidomethyl (C)
Variable modifications: 
Y9     : Phospho (Y)
R13    : Arginine-13C615N4 (R_10)
Ions Score: 71  Expect: 4.6e-05  
Matches (Bold Red): 22/138 fragment ions using 26 most intense peaks

#	b	b⁺⁺	b*	b*⁺⁺	b⁰	b⁰⁺⁺	Seq.	y	y⁺⁺	y*	y*⁺⁺	y⁰	y⁰⁺⁺	#
1	129.0659	65.0366	112.0393	56.5233			Q							13
2	200.1030	100.5551	183.0764	92.0418			A	1418.5897	709.7985	1401.5631	701.2852	1400.5791	700.7932	12
3	315.1299	158.0686	298.1034	149.5553	297.1193	149.0633	D	1347.5526	674.2799	1330.5260	665.7666	1329.5420	665.2746	11
4	402.1619	201.5846	385.1354	193.0713	384.1514	192.5793	S	1232.5256	616.7665	1215.4991	608.2532	1214.5151	607.7612	10
5	531.2045	266.1059	514.1780	257.5926	513.1940	257.1006	E	1145.4936	573.2504	1128.4671	564.7372	1127.4830	564.2452	9
6	662.2450	331.6261	645.2185	323.1129	644.2344	322.6209	M	1016.4510	508.7291	999.4245	500.2159	998.4404	499.7239	8
7	763.2927	382.1500	746.2661	373.6367	745.2821	373.1447	T	885.4105	443.2089	868.3840	434.6956	867.4000	434.2036	7
8	820.3141	410.6607	803.2876	402.1474	802.3036	401.6554	G	784.3629	392.6851	767.3363	384.1718	766.3523	383.6798	6
9	1063.3438	532.1755	1046.3173	523.6623	1045.3332	523.1703	Y	727.3414	364.1743	710.3148	355.6611	709.3308	355.1691	5
10	1162.4122	581.7097	1145.3857	573.1965	1144.4016	572.7045	V	484.3117	242.6595	467.2852	234.1462	466.3012	233.6542	4
11	1261.4806	631.2439	1244.4541	622.7307	1243.4701	622.2387	V	385.2433	193.1253	368.2168	184.6120	367.2328	184.1200	3
12	1362.5283	681.7678	1345.5018	673.2545	1344.5177	672.7625	T	286.1749	143.5911	269.1484	135.0778	268.1644	134.5858	2
13							R	185.1272	93.0673	168.1007	84.5540			1

Error Distribution Error Distribution (ppm)

NCBI BLAST search of QADSEMTGYVVTR
(Parameters: blastp, nr protein database, expect=20000, no filter, PAM30)
Other BLAST web gateways

All matches to this query

Score Mr(calc): Delta Sequence

71.2 1545.6410 0.0085 QADSEMTGYVVTR

10.2 1545.6351 0.0144 AFSRSSSLTRHK

9.6 1545.6353 0.0142 RETETERQEQR

9.6 1545.6353 0.0142 RETETERQEQR

6.5 1545.6413 0.0081 KGLSNYAVTFQR

4.7 1545.6615 -0.0121 TDATFQSFMKLR

4.6 1545.6409 0.0085 MFDSKEKASEER

4.3 1545.6442 0.0053 FREQRYSLFK

3.9 1545.6661 -0.0167 SFSEMLQNLDKK

3.9 1545.6661 -0.0167 SFSEMLQNLDKK

Score	Mr(calc):	Delta	Sequence
71.2	1545.6410	0.0085	QADSEMTGYVVTR
10.2	1545.6351	0.0144	AFSRSSSLTRHK
9.6	1545.6353	0.0142	RETETERQEQR
9.6	1545.6353	0.0142	RETETERQEQR
6.5	1545.6413	0.0081	KGLSNYAVTFQR
4.7	1545.6615	-0.0121	TDATFQSFMKLR
4.6	1545.6409	0.0085	MFDSKEKASEER
4.3	1545.6442	0.0053	FREQRYSLFK
3.9	1545.6661	-0.0167	SFSEMLQNLDKK
3.9	1545.6661	-0.0167	SFSEMLQNLDKK

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