Mascot Search Results

Peptide View

MS/MS Fragmentation of VADPDHDHTGFLTEYVATR
Found in ENSP00000215832, gene:MAPK1 -- Mitogen-activated protein kinase 1 (EC 2.7.11.24) (Extracellular signal-regulated k

Match to Query 7190: 2306.928111 from(769.983313,3+)
Title: Elution from: 777.777 to 777.777 period: 091207_bidira_10min_2e6_4g10.raw experiment: 1 cycles: 1 precIntensity: 243552.0 FinneganScanNumber: 4908
Data file 091207_BidirA_b1_10min_conj.msm

Monoisotopic mass of neutral peptide Mr(calc): 2306.9178
Fixed modifications: Carbamidomethyl (C)
Variable modifications: 
T13    : Phospho (ST), with neutral loss 97.9769
Y15    : Phospho (Y)
R19    : Arginine-15N4 (R_4)
Ions Score: 47  Expect: 0.043  
Matches (Bold Red): 27/174 fragment ions using 38 most intense peaks

#	b	b⁺⁺	b⁰	b⁰⁺⁺	Seq.	y	y⁺⁺	y*	y*⁺⁺	y⁰	y⁰⁺⁺	#
1	100.0757	50.5415			V							19
2	171.1128	86.0600			A	2110.8798	1055.9435	2093.8532	1047.4303	2092.8692	1046.9383	18
3	286.1397	143.5735	268.1292	134.5682	D	2039.8427	1020.4250	2022.8161	1011.9117	2021.8321	1011.4197	17
4	383.1925	192.0999	365.1819	183.0946	P	1924.8157	962.9115	1907.7892	954.3982	1906.8052	953.9062	16
5	498.2194	249.6134	480.2089	240.6081	D	1827.7630	914.3851	1810.7364	905.8719	1809.7524	905.3798	15
6	635.2783	318.1428	617.2678	309.1375	H	1712.7360	856.8717	1695.7095	848.3584	1694.7255	847.8664	14
7	750.3053	375.6563	732.2947	366.6510	D	1575.6771	788.3422	1558.6506	779.8289	1557.6666	779.3369	13
8	887.3642	444.1857	869.3536	435.1805	H	1460.6502	730.8287	1443.6236	722.3155	1442.6396	721.8235	12
9	988.4119	494.7096	970.4013	485.7043	T	1323.5913	662.2993	1306.5647	653.7860	1305.5807	653.2940	11
10	1045.4333	523.2203	1027.4228	514.2150	G	1222.5436	611.7754	1205.5171	603.2622	1204.5330	602.7702	10
11	1192.5017	596.7545	1174.4912	587.7492	F	1165.5221	583.2647	1148.4956	574.7514	1147.5116	574.2594	9
12	1305.5858	653.2965	1287.5752	644.2913	L	1018.4537	509.7305	1001.4272	501.2172	1000.4432	500.7252	8
13	1388.6229	694.8151	1370.6123	685.8098	T	905.3697	453.1885	888.3431	444.6752	887.3591	444.1832	7
14	1517.6655	759.3364	1499.6549	750.3311	E	822.3326	411.6699	805.3060	403.1566	804.3220	402.6646	6
15	1760.6952	880.8512	1742.6846	871.8459	Y	693.2900	347.1486	676.2634	338.6354	675.2794	338.1433	5
16	1859.7636	930.3854	1841.7530	921.3801	V	450.2603	225.6338	433.2338	217.1205	432.2498	216.6285	4
17	1930.8007	965.9040	1912.7901	956.8987	A	351.1919	176.0996	334.1654	167.5863	333.1814	167.0943	3
18	2031.8484	1016.4278	2013.8378	1007.4225	T	280.1548	140.5810	263.1283	132.0678	262.1442	131.5758	2
19					R	179.1071	90.0572	162.0806	81.5439			1

Error Distribution Error Distribution (ppm)

NCBI BLAST search of VADPDHDHTGFLTEYVATR
(Parameters: blastp, nr protein database, expect=20000, no filter, PAM30)
Other BLAST web gateways

All matches to this query

Score Mr(calc): Delta Sequence

47.2 2306.9178 0.0103 VADPDHDHTGFLTEYVATR

27.3 2306.9178 0.0103 VADPDHDHTGFLTEYVATR

20.5 2306.9178 0.0103 VADPDHDHTGFLTEYVATR

8.5 2306.9084 0.0197 MSGSASSENREGTLSDSTGSEK

6.7 2306.9093 0.0189 GRSGNGNEPQGSPTEIPFSSK

5.6 2306.9084 0.0197 MSGSASSENREGTLSDSTGSEK

5.5 2306.9158 0.0123 IGDTSVSYKYTSRYVLK

5.2 2306.9084 0.0197 MSGSASSENREGTLSDSTGSEK

5.1 2306.9396 -0.0115 LPNTKMRSSNSASASAGPPGK

4.5 2306.9671 -0.0390 ANAPPTPLPMHSSKAPAYNR

Score	Mr(calc):	Delta	Sequence
47.2	2306.9178	0.0103	VADPDHDHTGFLTEYVATR
27.3	2306.9178	0.0103	VADPDHDHTGFLTEYVATR
20.5	2306.9178	0.0103	VADPDHDHTGFLTEYVATR
8.5	2306.9084	0.0197	MSGSASSENREGTLSDSTGSEK
6.7	2306.9093	0.0189	GRSGNGNEPQGSPTEIPFSSK
5.6	2306.9084	0.0197	MSGSASSENREGTLSDSTGSEK
5.5	2306.9158	0.0123	IGDTSVSYKYTSRYVLK
5.2	2306.9084	0.0197	MSGSASSENREGTLSDSTGSEK
5.1	2306.9396	-0.0115	LPNTKMRSSNSASASAGPPGK
4.5	2306.9671	-0.0390	ANAPPTPLPMHSSKAPAYNR

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