Mascot Search Results

Peptide View

MS/MS Fragmentation of VADPDHDHTGFLTEYVATR
Found in ENSP00000215832, gene:MAPK1 -- Mitogen-activated protein kinase 1 (EC 2.7.11.24) (Extracellular signal-regulated k

Match to Query 29200: 2312.945856 from(1157.480204,2+)
Title: Elution from: 777.777 to 777.777 period: 100308_b2dw_b1fc_5min_4g10_ip1.raw experiment: 1 cycles: 1 precIntensity: 82744.0 FinneganScanNumber: 7487
Data file 100308_DWB2_b1Fc_5min_conj.msm

Monoisotopic mass of neutral peptide Mr(calc): 2312.9379
Fixed modifications: Carbamidomethyl (C)
Variable modifications: 
T13    : Phospho (ST), with neutral loss 97.9769
Y15    : Phospho (Y)
R19    : Arginine-13C615N4 (R_10)
Ions Score: 58  Expect: 0.0027  
Matches (Bold Red): 45/174 fragment ions using 70 most intense peaks

#	b	b⁺⁺	b⁰	b⁰⁺⁺	Seq.	y	y⁺⁺	y*	y*⁺⁺	y⁰	y⁰⁺⁺	#
1	100.0757	50.5415			V							19
2	171.1128	86.0600			A	2116.8999	1058.9536	2099.8734	1050.4403	2098.8893	1049.9483	18
3	286.1397	143.5735	268.1292	134.5682	D	2045.8628	1023.4350	2028.8363	1014.9218	2027.8522	1014.4298	17
4	383.1925	192.0999	365.1819	183.0946	P	1930.8359	965.9216	1913.8093	957.4083	1912.8253	956.9163	16
5	498.2194	249.6134	480.2089	240.6081	D	1833.7831	917.3952	1816.7566	908.8819	1815.7725	908.3899	15
6	635.2783	318.1428	617.2678	309.1375	H	1718.7562	859.8817	1701.7296	851.3684	1700.7456	850.8764	14
7	750.3053	375.6563	732.2947	366.6510	D	1581.6972	791.3523	1564.6707	782.8390	1563.6867	782.3470	13
8	887.3642	444.1857	869.3536	435.1805	H	1466.6703	733.8388	1449.6438	725.3255	1448.6597	724.8335	12
9	988.4119	494.7096	970.4013	485.7043	T	1329.6114	665.3093	1312.5849	656.7961	1311.6008	656.3041	11
10	1045.4333	523.2203	1027.4228	514.2150	G	1228.5637	614.7855	1211.5372	606.2722	1210.5532	605.7802	10
11	1192.5017	596.7545	1174.4912	587.7492	F	1171.5423	586.2748	1154.5157	577.7615	1153.5317	577.2695	9
12	1305.5858	653.2965	1287.5752	644.2913	L	1024.4738	512.7406	1007.4473	504.2273	1006.4633	503.7353	8
13	1388.6229	694.8151	1370.6123	685.8098	T	911.3898	456.1985	894.3632	447.6853	893.3792	447.1933	7
14	1517.6655	759.3364	1499.6549	750.3311	E	828.3527	414.6800	811.3261	406.1667	810.3421	405.6747	6
15	1760.6952	880.8512	1742.6846	871.8459	Y	699.3101	350.1587	682.2835	341.6454	681.2995	341.1534	5
16	1859.7636	930.3854	1841.7530	921.3801	V	456.2804	228.6439	439.2539	220.1306	438.2699	219.6386	4
17	1930.8007	965.9040	1912.7901	956.8987	A	357.2120	179.1097	340.1855	170.5964	339.2015	170.1044	3
18	2031.8484	1016.4278	2013.8378	1007.4225	T	286.1749	143.5911	269.1484	135.0778	268.1644	134.5858	2
19					R	185.1272	93.0673	168.1007	84.5540			1

Error Distribution Error Distribution (ppm)

NCBI BLAST search of VADPDHDHTGFLTEYVATR
(Parameters: blastp, nr protein database, expect=20000, no filter, PAM30)
Other BLAST web gateways

All matches to this query

Score Mr(calc): Delta Sequence

57.5 2312.9379 0.0079 VADPDHDHTGFLTEYVATR

37.3 2312.9379 0.0079 VADPDHDHTGFLTEYVATR

27.6 2312.9379 0.0079 VADPDHDHTGFLTEYVATR

14.2 2312.9379 0.0079 VADPDHDHTGFLTEYVATR

7.9 2312.9379 0.0079 VADPDHDHTGFLTEYVATR

7.8 2312.9230 0.0228 GQTSNTKLLGCQSIEKFR

7.8 2312.9230 0.0228 GQTSNTKLLGCQSIEKFR

7.5 2312.9230 0.0228 GQTSNTKLLGCQSIEKFR

7.5 2312.9230 0.0228 GQTSNTKLLGCQSIEKFR

6.1 2312.9614 -0.0155 LGPNDQYKFYSVNVDYSK

Score	Mr(calc):	Delta	Sequence
57.5	2312.9379	0.0079	VADPDHDHTGFLTEYVATR
37.3	2312.9379	0.0079	VADPDHDHTGFLTEYVATR
27.6	2312.9379	0.0079	VADPDHDHTGFLTEYVATR
14.2	2312.9379	0.0079	VADPDHDHTGFLTEYVATR
7.9	2312.9379	0.0079	VADPDHDHTGFLTEYVATR
7.8	2312.9230	0.0228	GQTSNTKLLGCQSIEKFR
7.8	2312.9230	0.0228	GQTSNTKLLGCQSIEKFR
7.5	2312.9230	0.0228	GQTSNTKLLGCQSIEKFR
7.5	2312.9230	0.0228	GQTSNTKLLGCQSIEKFR
6.1	2312.9614	-0.0155	LGPNDQYKFYSVNVDYSK

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