Mascot Search Results

Peptide View

MS/MS Fragmentation of ELEQVCNPIISGLYQGAGGPGPGGFGAQGPK
Found in ENSP00000364801, gene:HSPA1B -- Heat shock 70 kDa protein 1 (HSP70.1) (HSP70-1/HSP70-2). [Source:Uniprot/SWISSPROT

Match to Query 33541: 3142.482744 from(1048.501524,3+)
Title: Elution from: 777.777 to 777.777 period: 100308_b2dw_b1fc_5min_ptyr100_ip1.raw experiment: 1 cycles: 1 precIntensity: 69186.0 FinneganScanNumber: 9838
Data file 100308_DWB2_b1Fc_5min_conj.msm

Monoisotopic mass of neutral peptide Mr(calc): 3142.4673
Fixed modifications: Carbamidomethyl (C)
Variable modifications: 
Y14    : Phospho (Y)
K31    : Lysine-13C615N2 (K_8)
Ions Score: 42  Expect: 0.19  
Matches (Bold Red): 23/314 fragment ions using 40 most intense peaks

#	b	b⁺⁺	b*	b*⁺⁺	b⁰	b⁰⁺⁺	Seq.	y	y⁺⁺	y*	y*⁺⁺	y⁰	y⁰⁺⁺	#
1	130.0499	65.5286			112.0393	56.5233	E							31
2	243.1339	122.0706			225.1234	113.0653	L	3014.4321	1507.7197	2997.4055	1499.2064	2996.4215	1498.7144	30
3	372.1765	186.5919			354.1660	177.5866	E	2901.3480	1451.1776	2884.3215	1442.6644	2883.3374	1442.1724	29
4	500.2351	250.6212	483.2085	242.1079	482.2245	241.6159	Q	2772.3054	1386.6563	2755.2789	1378.1431	2754.2948	1377.6511	28
5	599.3035	300.1554	582.2770	291.6421	581.2929	291.1501	V	2644.2468	1322.6271	2627.2203	1314.1138	2626.2363	1313.6218	27
6	759.3342	380.1707	742.3076	371.6574	741.3236	371.1654	C	2545.1784	1273.0928	2528.1519	1264.5796	2527.1679	1264.0876	26
7	873.3771	437.1922	856.3505	428.6789	855.3665	428.1869	N	2385.1478	1193.0775	2368.1212	1184.5642	2367.1372	1184.0722	25
8	970.4298	485.7186	953.4033	477.2053	952.4193	476.7133	P	2271.1048	1136.0561	2254.0783	1127.5428	2253.0943	1127.0508	24
9	1083.5139	542.2606	1066.4874	533.7473	1065.5033	533.2553	I	2174.0521	1087.5297	2157.0255	1079.0164	2156.0415	1078.5244	23
10	1196.5980	598.8026	1179.5714	590.2893	1178.5874	589.7973	I	2060.9680	1030.9876	2043.9415	1022.4744	2042.9575	1021.9824	22
11	1283.6300	642.3186	1266.6034	633.8054	1265.6194	633.3134	S	1947.8840	974.4456	1930.8574	965.9323	1929.8734	965.4403	21
12	1340.6515	670.8294	1323.6249	662.3161	1322.6409	661.8241	G	1860.8519	930.9296	1843.8254	922.4163			20
13	1453.7355	727.3714	1436.7090	718.8581	1435.7249	718.3661	L	1803.8305	902.4189	1786.8039	893.9056			19
14	1696.7652	848.8862	1679.7386	840.3729	1678.7546	839.8809	Y	1690.7464	845.8768	1673.7199	837.3636			18
15	1824.8237	912.9155	1807.7972	904.4022	1806.8132	903.9102	Q	1447.7168	724.3620	1430.6902	715.8487			17
16	1881.8452	941.4262	1864.8187	932.9130	1863.8346	932.4210	G	1319.6582	660.3327	1302.6316	651.8195			16
17	1952.8823	976.9448	1935.8558	968.4315	1934.8718	967.9395	A	1262.6367	631.8220	1245.6102	623.3087			15
18	2009.9038	1005.4555	1992.8772	996.9423	1991.8932	996.4502	G	1191.5996	596.3034	1174.5731	587.7902			14
19	2066.9252	1033.9663	2049.8987	1025.4530	2048.9147	1024.9610	G	1134.5781	567.7927	1117.5516	559.2794			13
20	2163.9780	1082.4926	2146.9514	1073.9794	2145.9674	1073.4874	P	1077.5567	539.2820	1060.5301	530.7687			12
21	2220.9995	1111.0034	2203.9729	1102.4901	2202.9889	1101.9981	G	980.5039	490.7556	963.4774	482.2423			11
22	2318.0522	1159.5297	2301.0257	1151.0165	2300.0417	1150.5245	P	923.4825	462.2449	906.4559	453.7316			10
23	2375.0737	1188.0405	2358.0471	1179.5272	2357.0631	1179.0352	G	826.4297	413.7185	809.4032	405.2052			9
24	2432.0951	1216.5512	2415.0686	1208.0379	2414.0846	1207.5459	G	769.4082	385.2078	752.3817	376.6945			8
25	2579.1635	1290.0854	2562.1370	1281.5721	2561.1530	1281.0801	F	712.3868	356.6970	695.3602	348.1838			7
26	2636.1850	1318.5961	2619.1585	1310.0829	2618.1744	1309.5909	G	565.3184	283.1628	548.2918	274.6496			6
27	2707.2221	1354.1147	2690.1956	1345.6014	2689.2116	1345.1094	A	508.2969	254.6521	491.2704	246.1388			5
28	2835.2807	1418.1440	2818.2541	1409.6307	2817.2701	1409.1387	Q	437.2598	219.1335	420.2333	210.6203			4
29	2892.3022	1446.6547	2875.2756	1438.1414	2874.2916	1437.6494	G	309.2012	155.1043	292.1747	146.5910			3
30	2989.3549	1495.1811	2972.3284	1486.6678	2971.3444	1486.1758	P	252.1798	126.5935	235.1532	118.0802			2
31							K	155.1270	78.0671	138.1005	69.5539			1

Error Distribution Error Distribution (ppm)

NCBI BLAST search of ELEQVCNPIISGLYQGAGGPGPGGFGAQGPK
(Parameters: blastp, nr protein database, expect=20000, no filter, PAM30)
Other BLAST web gateways

All matches to this query

Score Mr(calc): Delta Sequence

42.0 3142.4673 0.0154 ELEQVCNPIISGLYQGAGGPGPGGFGAQGPK

11.0 3142.4673 0.0154 ELEQVCNPIISGLYQGAGGPGPGGFGAQGPK

7.7 3142.4725 0.0103 MGWQRLLLLPRPPASTGASNATRRPK

7.7 3142.4725 0.0103 MGWQRLLLLPRPPASTGASNATRRPK

7.7 3142.4725 0.0103 MGWQRLLLLPRPPASTGASNATRRPK

7.7 3142.4725 0.0103 MGWQRLLLLPRPPASTGASNATRRPK

7.7 3142.4879 -0.0052 LKIKETVINGLENMGAAFQSMMTGGNIGK

7.7 3142.4558 0.0269 TCEKRPTFNQHTVFNLHQRLNTGDK

5.9 3142.4462 0.0366 GPPPLPTDMRQISQDFSELSTQLTGVAR

5.9 3142.4462 0.0366 GPPPLPTDMRQISQDFSELSTQLTGVAR

Score	Mr(calc):	Delta	Sequence
42.0	3142.4673	0.0154	ELEQVCNPIISGLYQGAGGPGPGGFGAQGPK
11.0	3142.4673	0.0154	ELEQVCNPIISGLYQGAGGPGPGGFGAQGPK
7.7	3142.4725	0.0103	MGWQRLLLLPRPPASTGASNATRRPK
7.7	3142.4725	0.0103	MGWQRLLLLPRPPASTGASNATRRPK
7.7	3142.4725	0.0103	MGWQRLLLLPRPPASTGASNATRRPK
7.7	3142.4725	0.0103	MGWQRLLLLPRPPASTGASNATRRPK
7.7	3142.4879	-0.0052	LKIKETVINGLENMGAAFQSMMTGGNIGK
7.7	3142.4558	0.0269	TCEKRPTFNQHTVFNLHQRLNTGDK
5.9	3142.4462	0.0366	GPPPLPTDMRQISQDFSELSTQLTGVAR
5.9	3142.4462	0.0366	GPPPLPTDMRQISQDFSELSTQLTGVAR

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