Mascot Search Results

Peptide View

MS/MS Fragmentation of ETAPTSAYSSPAR
Found in ENSP00000298777, gene:HSPA12A -- Heat shock 70 kDa protein 12A. [Source:Uniprot/SWISSPROT;Acc:O43301]

Match to Query 7658: 1416.610518 from(709.312535,2+)
Title: Elution from: 777.777 to 777.777 period: 021107_dwbidira_py100_ip2.raw experiment: 1 cycles: 1 precIntensity: 11474.0 FinneganScanNumber: 2792
Data file 021107_BidirA_B2DW_10min_conj_2.msm

Monoisotopic mass of neutral peptide Mr(calc): 1416.5922
Fixed modifications: Carbamidomethyl (C)
Variable modifications: 
Y8     : Phospho (Y)
Ions Score: 55  Expect: 0.0029  
Matches (Bold Red): 17/114 fragment ions using 24 most intense peaks

#	b	b⁺⁺	b⁰	b⁰⁺⁺	Seq.	y	y⁺⁺	y*	y*⁺⁺	y⁰	y⁰⁺⁺	#
1	130.0499	65.5286	112.0393	56.5233	E							13
2	231.0975	116.0524	213.0870	107.0471	T	1288.5569	644.7821	1271.5304	636.2688	1270.5463	635.7768	12
3	302.1347	151.5710	284.1241	142.5657	A	1187.5092	594.2583	1170.4827	585.7450	1169.4987	585.2530	11
4	399.1874	200.0973	381.1769	191.0921	P	1116.4721	558.7397	1099.4456	550.2264	1098.4615	549.7344	10
5	500.2351	250.6212	482.2245	241.6159	T	1019.4194	510.2133	1002.3928	501.7000	1001.4088	501.2080	9
6	587.2671	294.1372	569.2566	285.1319	S	918.3717	459.6895	901.3451	451.1762	900.3611	450.6842	8
7	658.3042	329.6558	640.2937	320.6505	A	831.3396	416.1735	814.3131	407.6602	813.3291	407.1682	7
8	901.3339	451.1706	883.3233	442.1653	Y	760.3025	380.6549	743.2760	372.1416	742.2920	371.6496	6
9	988.3659	494.6866	970.3554	485.6813	S	517.2729	259.1401	500.2463	250.6268	499.2623	250.1348	5
10	1075.3979	538.2026	1057.3874	529.1973	S	430.2409	215.6241	413.2143	207.1108	412.2303	206.6188	4
11	1172.4507	586.7290	1154.4401	577.7237	P	343.2088	172.1080	326.1823	163.5948			3
12	1243.4878	622.2475	1225.4773	613.2423	A	246.1561	123.5817	229.1295	115.0684			2
13					R	175.1190	88.0631	158.0924	79.5498			1

Error Distribution Error Distribution (ppm)

NCBI BLAST search of ETAPTSAYSSPAR
(Parameters: blastp, nr protein database, expect=20000, no filter, PAM30)
Other BLAST web gateways

All matches to this query

Score Mr(calc): Delta Sequence

55.1 1416.5922 0.0183 ETAPTSAYSSPAR

8.6 1416.5956 0.0150 MISEVDREGTGK

8.5 1416.5995 0.0110 WYQQWKDFK

8.4 1416.5835 0.0270 TSGNSFKVNSCK

8.4 1416.5835 0.0270 TSGNSFKVNSCK

7.2 1416.6157 -0.0052 AVMYLYKHPK

7.2 1416.6157 -0.0052 AVMYLYKHPK

6.5 1416.6108 -0.0003 QTAKYMHKDAK

4.2 1416.6286 -0.0180 GENEKIVETYR

4.2 1416.6286 -0.0180 GENEKIVETYR

Score	Mr(calc):	Delta	Sequence
55.1	1416.5922	0.0183	ETAPTSAYSSPAR
8.6	1416.5956	0.0150	MISEVDREGTGK
8.5	1416.5995	0.0110	WYQQWKDFK
8.4	1416.5835	0.0270	TSGNSFKVNSCK
8.4	1416.5835	0.0270	TSGNSFKVNSCK
7.2	1416.6157	-0.0052	AVMYLYKHPK
7.2	1416.6157	-0.0052	AVMYLYKHPK
6.5	1416.6108	-0.0003	QTAKYMHKDAK
4.2	1416.6286	-0.0180	GENEKIVETYR
4.2	1416.6286	-0.0180	GENEKIVETYR

Mascot: http://www.matrixscience.com/