Mascot Search Results

Peptide View

MS/MS Fragmentation of ETAPTSAYSSPAR
Found in ENSP00000298777, gene:HSPA12A -- Heat shock 70 kDa protein 12A. [Source:Uniprot/SWISSPROT;Acc:O43301]

Match to Query 13515: 1416.593892 from(709.304222,2+)
Title: Elution from: 777.777 to 777.777 period: 230208_b2dw_b1fc_5min_4g10_ip2.raw experiment: 1 cycles: 1 precIntensity: 16080.0 FinneganScanNumber: 2700
Data file 230208_DWB2_b1Fc_5min_conj.msm

Monoisotopic mass of neutral peptide Mr(calc): 1416.5922
Fixed modifications: Carbamidomethyl (C)
Variable modifications: 
Y8     : Phospho (Y)
Ions Score: 49  Expect: 0.0095  
Matches (Bold Red): 12/114 fragment ions using 15 most intense peaks

#	b	b⁺⁺	b⁰	b⁰⁺⁺	Seq.	y	y⁺⁺	y*	y*⁺⁺	y⁰	y⁰⁺⁺	#
1	130.0499	65.5286	112.0393	56.5233	E							13
2	231.0975	116.0524	213.0870	107.0471	T	1288.5569	644.7821	1271.5304	636.2688	1270.5463	635.7768	12
3	302.1347	151.5710	284.1241	142.5657	A	1187.5092	594.2583	1170.4827	585.7450	1169.4987	585.2530	11
4	399.1874	200.0973	381.1769	191.0921	P	1116.4721	558.7397	1099.4456	550.2264	1098.4615	549.7344	10
5	500.2351	250.6212	482.2245	241.6159	T	1019.4194	510.2133	1002.3928	501.7000	1001.4088	501.2080	9
6	587.2671	294.1372	569.2566	285.1319	S	918.3717	459.6895	901.3451	451.1762	900.3611	450.6842	8
7	658.3042	329.6558	640.2937	320.6505	A	831.3396	416.1735	814.3131	407.6602	813.3291	407.1682	7
8	901.3339	451.1706	883.3233	442.1653	Y	760.3025	380.6549	743.2760	372.1416	742.2920	371.6496	6
9	988.3659	494.6866	970.3554	485.6813	S	517.2729	259.1401	500.2463	250.6268	499.2623	250.1348	5
10	1075.3979	538.2026	1057.3874	529.1973	S	430.2409	215.6241	413.2143	207.1108	412.2303	206.6188	4
11	1172.4507	586.7290	1154.4401	577.7237	P	343.2088	172.1080	326.1823	163.5948			3
12	1243.4878	622.2475	1225.4773	613.2423	A	246.1561	123.5817	229.1295	115.0684			2
13					R	175.1190	88.0631	158.0924	79.5498			1

Error Distribution Error Distribution (ppm)

NCBI BLAST search of ETAPTSAYSSPAR
(Parameters: blastp, nr protein database, expect=20000, no filter, PAM30)
Other BLAST web gateways

All matches to this query

Score Mr(calc): Delta Sequence

49.4 1416.5922 0.0017 ETAPTSAYSSPAR

11.6 1416.5785 0.0154 MAASPGSGSANPRK

11.6 1416.5785 0.0154 MAASPGSGSANPRK

8.7 1416.6216 -0.0278 REGSGCLAADNMK

7.5 1416.5835 0.0103 TSGNSFKVNSCK

7.5 1416.5835 0.0103 TSGNSFKVNSCK

4.9 1416.5666 0.0273 SNTDVSLKQEK

4.5 1416.5814 0.0125 SRAKENAEDAGGK

3.6 1416.6024 -0.0085 DWLYKCSVQK

3.4 1416.5668 0.0271 AIMYEETTYSK

Score	Mr(calc):	Delta	Sequence
49.4	1416.5922	0.0017	ETAPTSAYSSPAR
11.6	1416.5785	0.0154	MAASPGSGSANPRK
11.6	1416.5785	0.0154	MAASPGSGSANPRK
8.7	1416.6216	-0.0278	REGSGCLAADNMK
7.5	1416.5835	0.0103	TSGNSFKVNSCK
7.5	1416.5835	0.0103	TSGNSFKVNSCK
4.9	1416.5666	0.0273	SNTDVSLKQEK
4.5	1416.5814	0.0125	SRAKENAEDAGGK
3.6	1416.6024	-0.0085	DWLYKCSVQK
3.4	1416.5668	0.0271	AIMYEETTYSK

Mascot: http://www.matrixscience.com/