Mascot Search Results

Peptide View

MS/MS Fragmentation of ISGLIYEETR
Found in ENSP00000244484, gene:HIST1H4B -- Histone H4. [Source:Uniprot/SWISSPROT;Acc:P62805]

Match to Query 10563: 1259.584270 from(630.799411,2+)
Title: Elution from: 777.777 to 777.777 period: 230208_dwb2_b1fc_5min_ptyr100_ip1.raw experiment: 1 cycles: 1 precIntensity: 27105.0 FinneganScanNumber: 4620
Data file 230208_DWB2_b1Fc_5min_conj.msm

Monoisotopic mass of neutral peptide Mr(calc): 1259.5798
Fixed modifications: Carbamidomethyl (C)
Variable modifications: 
Y6     : Phospho (Y)
Ions Score: 54  Expect: 0.0032  
Matches (Bold Red): 26/86 fragment ions using 33 most intense peaks

#	b	b⁺⁺	b⁰	b⁰⁺⁺	Seq.	y	y⁺⁺	y*	y*⁺⁺	y⁰	y⁰⁺⁺	#
1	114.0913	57.5493			I							10
2	201.1234	101.0653	183.1128	92.0600	S	1147.5031	574.2552	1130.4765	565.7419	1129.4925	565.2499	9
3	258.1448	129.5761	240.1343	120.5708	G	1060.4710	530.7392	1043.4445	522.2259	1042.4605	521.7339	8
4	371.2289	186.1181	353.2183	177.1128	L	1003.4496	502.2284	986.4230	493.7152	985.4390	493.2231	7
5	484.3129	242.6601	466.3024	233.6548	I	890.3655	445.6864	873.3390	437.1731	872.3550	436.6811	6
6	727.3426	364.1749	709.3320	355.1697	Y	777.2815	389.1444	760.2549	380.6311	759.2709	380.1391	5
7	856.3852	428.6962	838.3746	419.6910	E	534.2518	267.6295	517.2253	259.1163	516.2412	258.6243	4
8	985.4278	493.2175	967.4172	484.2122	E	405.2092	203.1082	388.1827	194.5950	387.1987	194.1030	3
9	1086.4755	543.7414	1068.4649	534.7361	T	276.1666	138.5870	259.1401	130.0737	258.1561	129.5817	2
10					R	175.1190	88.0631	158.0924	79.5498			1

Error Distribution Error Distribution (ppm)

NCBI BLAST search of ISGLIYEETR
(Parameters: blastp, nr protein database, expect=20000, no filter, PAM30)
Other BLAST web gateways

All matches to this query

Score Mr(calc): Delta Sequence

54.4 1259.5798 0.0044 ISGLIYEETR

14.1 1259.5831 0.0012 LSGLQSRYSR

13.2 1259.6002 -0.0159 EKMQMELSHK

12.4 1259.5831 0.0012 LSGLQSRYSR

10.1 1259.6021 -0.0179 LDLAAYDQEGR

10.1 1259.5691 0.0151 IQELMSDDQR

9.9 1259.5877 -0.0035 QKLKASENSR

9.5 1259.6095 -0.0252 ISIGSYPPSKK

9.2 1259.5674 0.0169 ERGHKDQEEK

8.1 1259.5907 -0.0064 MEQQRSQLSK

Score	Mr(calc):	Delta	Sequence
54.4	1259.5798	0.0044	ISGLIYEETR
14.1	1259.5831	0.0012	LSGLQSRYSR
13.2	1259.6002	-0.0159	EKMQMELSHK
12.4	1259.5831	0.0012	LSGLQSRYSR
10.1	1259.6021	-0.0179	LDLAAYDQEGR
10.1	1259.5691	0.0151	IQELMSDDQR
9.9	1259.5877	-0.0035	QKLKASENSR
9.5	1259.6095	-0.0252	ISIGSYPPSKK
9.2	1259.5674	0.0169	ERGHKDQEEK
8.1	1259.5907	-0.0064	MEQQRSQLSK

Mascot: http://www.matrixscience.com/