Mascot Search Results

Peptide View

MS/MS Fragmentation of LDDQSEDEALELEGPLITPGSGSIYSSPGLYSK
Found in ENSP00000342858, gene:PLEKHC1 -- Pleckstrin homology domain-containing family C member 1 (Kindlin-2) (Mitogen-indu

Match to Query 8075: 3546.625842 from(1183.215890,3+)
Title: Elution from: 777.777 to 777.777 period: 091207_bidira_10min_2e6_4g10.raw experiment: 1 cycles: 1 precIntensity: 20735.0 FinneganScanNumber: 8777
Data file 091207_BidirA_b1_10min_conj.msm

Monoisotopic mass of neutral peptide Mr(calc): 3546.6178
Fixed modifications: Carbamidomethyl (C)
Variable modifications: 
Y25    : Phospho (Y)
Ions Score: 52  Expect: 0.036  
Matches (Bold Red): 46/374 fragment ions using 67 most intense peaks

#	b	b⁺⁺	b*	b*⁺⁺	b⁰	b⁰⁺⁺	Seq.	y	y⁺⁺	y*	y*⁺⁺	y⁰	y⁰⁺⁺	#
1	114.0913	57.5493					L							33
2	229.1183	115.0628			211.1077	106.0575	D	3434.5410	1717.7741	3417.5144	1709.2609	3416.5304	1708.7689	32
3	344.1452	172.5762			326.1347	163.5710	D	3319.5141	1660.2607	3302.4875	1651.7474	3301.5035	1651.2554	31
4	472.2038	236.6055	455.1772	228.0923	454.1932	227.6003	Q	3204.4871	1602.7472	3187.4606	1594.2339	3186.4765	1593.7419	30
5	559.2358	280.1216	542.2093	271.6083	541.2253	271.1163	S	3076.4285	1538.7179	3059.4020	1530.2046	3058.4180	1529.7126	29
6	688.2784	344.6428	671.2519	336.1296	670.2679	335.6376	E	2989.3965	1495.2019	2972.3700	1486.6886	2971.3859	1486.1966	28
7	803.3054	402.1563	786.2788	393.6430	785.2948	393.1510	D	2860.3539	1430.6806	2843.3274	1422.1673	2842.3433	1421.6753	27
8	932.3479	466.6776	915.3214	458.1643	914.3374	457.6723	E	2745.3270	1373.1671	2728.3004	1364.6539	2727.3164	1364.1618	26
9	1003.3851	502.1962	986.3585	493.6829	985.3745	493.1909	A	2616.2844	1308.6458	2599.2578	1300.1326	2598.2738	1299.6405	25
10	1116.4691	558.7382	1099.4426	550.2249	1098.4586	549.7329	L	2545.2473	1273.1273	2528.2207	1264.6140	2527.2367	1264.1220	24
11	1245.5117	623.2595	1228.4852	614.7462	1227.5011	614.2542	E	2432.1632	1216.5852	2415.1367	1208.0720	2414.1526	1207.5800	23
12	1358.5958	679.8015	1341.5692	671.2882	1340.5852	670.7962	L	2303.1206	1152.0640	2286.0941	1143.5507	2285.1101	1143.0587	22
13	1487.6384	744.3228	1470.6118	735.8095	1469.6278	735.3175	E	2190.0366	1095.5219	2173.0100	1087.0086	2172.0260	1086.5166	21
14	1544.6598	772.8335	1527.6333	764.3203	1526.6493	763.8283	G	2060.9940	1031.0006	2043.9674	1022.4874	2042.9834	1021.9953	20
15	1641.7126	821.3599	1624.6860	812.8467	1623.7020	812.3546	P	2003.9725	1002.4899	1986.9460	993.9766	1985.9619	993.4846	19
16	1754.7966	877.9020	1737.7701	869.3887	1736.7861	868.8967	L	1906.9198	953.9635	1889.8932	945.4502	1888.9092	944.9582	18
17	1867.8807	934.4440	1850.8541	925.9307	1849.8701	925.4387	I	1793.8357	897.4215	1776.8091	888.9082	1775.8251	888.4162	17
18	1968.9284	984.9678	1951.9018	976.4546	1950.9178	975.9625	T	1680.7516	840.8795	1663.7251	832.3662	1662.7411	831.8742	16
19	2065.9811	1033.4942	2048.9546	1024.9809	2047.9706	1024.4889	P	1579.7040	790.3556	1562.6774	781.8423	1561.6934	781.3503	15
20	2123.0026	1062.0049	2105.9760	1053.4917	2104.9920	1052.9997	G	1482.6512	741.8292	1465.6246	733.3160	1464.6406	732.8240	14
21	2210.0346	1105.5210	2193.0081	1097.0077	2192.0241	1096.5157	S	1425.6297	713.3185	1408.6032	704.8052	1407.6192	704.3132	13
22	2267.0561	1134.0317	2250.0295	1125.5184	2249.0455	1125.0264	G	1338.5977	669.8025	1321.5712	661.2892	1320.5871	660.7972	12
23	2354.0881	1177.5477	2337.0616	1169.0344	2336.0776	1168.5424	S	1281.5762	641.2918	1264.5497	632.7785	1263.5657	632.2865	11
24	2467.1722	1234.0897	2450.1456	1225.5765	2449.1616	1225.0844	I	1194.5442	597.7757	1177.5177	589.2625	1176.5336	588.7705	10
25	2710.2018	1355.6046	2693.1753	1347.0913	2692.1913	1346.5993	Y	1081.4602	541.2337	1064.4336	532.7204	1063.4496	532.2284	9
26	2797.2339	1399.1206	2780.2073	1390.6073	2779.2233	1390.1153	S	838.4305	419.7189	821.4040	411.2056	820.4199	410.7136	8
27	2884.2659	1442.6366	2867.2393	1434.1233	2866.2553	1433.6313	S	751.3985	376.2029	734.3719	367.6896	733.3879	367.1976	7
28	2981.3186	1491.1630	2964.2921	1482.6497	2963.3081	1482.1577	P	664.3664	332.6869	647.3399	324.1736	646.3559	323.6816	6
29	3038.3401	1519.6737	3021.3136	1511.1604	3020.3295	1510.6684	G	567.3137	284.1605	550.2871	275.6472	549.3031	275.1552	5
30	3151.4242	1576.2157	3134.3976	1567.7024	3133.4136	1567.2104	L	510.2922	255.6497	493.2657	247.1365	492.2817	246.6445	4
31	3314.4875	1657.7474	3297.4610	1649.2341	3296.4769	1648.7421	Y	397.2082	199.1077	380.1816	190.5944	379.1976	190.1024	3
32	3401.5195	1701.2634	3384.4930	1692.7501	3383.5090	1692.2581	S	234.1448	117.5761	217.1183	109.0628	216.1343	108.5708	2
33							K	147.1128	74.0600	130.0863	65.5468			1

Error Distribution Error Distribution (ppm)

NCBI BLAST search of LDDQSEDEALELEGPLITPGSGSIYSSPGLYSK
(Parameters: blastp, nr protein database, expect=20000, no filter, PAM30)
Other BLAST web gateways

All matches to this query

Score Mr(calc): Delta Sequence

52.4 3546.6178 0.0081 LDDQSEDEALELEGPLITPGSGSIYSSPGLYSK

50.0 3546.6178 0.0081 LDDQSEDEALELEGPLITPGSGSIYSSPGLYSK

18.1 3546.6965 -0.0707 GQVSVKVADVDLAVPVTHSCADLDSSNMLNTSSK

14.1 3546.6505 -0.0247 DIYQYLRQLEVLQSINPHFLDGRDINGR

14.1 3546.6505 -0.0247 DIYQYLRQLEVLQSINPHFLDGRDINGR

14.1 3546.5866 0.0393 DIYQYLRQLEVLQSINPHFLDGRDINGR

14.1 3546.5866 0.0393 DIYQYLRQLEVLQSINPHFLDGRDINGR

13.6 3546.6505 -0.0247 DIYQYLRQLEVLQSINPHFLDGRDINGR

13.6 3546.6505 -0.0247 DIYQYLRQLEVLQSINPHFLDGRDINGR

13.6 3546.5866 0.0393 DIYQYLRQLEVLQSINPHFLDGRDINGR

Score	Mr(calc):	Delta	Sequence
52.4	3546.6178	0.0081	LDDQSEDEALELEGPLITPGSGSIYSSPGLYSK
50.0	3546.6178	0.0081	LDDQSEDEALELEGPLITPGSGSIYSSPGLYSK
18.1	3546.6965	-0.0707	GQVSVKVADVDLAVPVTHSCADLDSSNMLNTSSK
14.1	3546.6505	-0.0247	DIYQYLRQLEVLQSINPHFLDGRDINGR
14.1	3546.6505	-0.0247	DIYQYLRQLEVLQSINPHFLDGRDINGR
14.1	3546.5866	0.0393	DIYQYLRQLEVLQSINPHFLDGRDINGR
14.1	3546.5866	0.0393	DIYQYLRQLEVLQSINPHFLDGRDINGR
13.6	3546.6505	-0.0247	DIYQYLRQLEVLQSINPHFLDGRDINGR
13.6	3546.6505	-0.0247	DIYQYLRQLEVLQSINPHFLDGRDINGR
13.6	3546.5866	0.0393	DIYQYLRQLEVLQSINPHFLDGRDINGR

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