Mascot Search Results

Peptide View

MS/MS Fragmentation of ADSEYTDKLQHYTSGHMTPGMK
Found in ENSP00000363758, gene:EPHB2 -- Ephrin type-B receptor 2 precursor (EC 2.7.10.1) (Tyrosine-protein kinase receptor

Match to Query 12857: 2576.075253 from(859.699027,3+)
Title: Elution from: 777.777 to 777.777 period: 070708_dwbidira_b1dv_10min_ip2.raw experiment: 1 cycles: 1 precIntensity: 25969.0 FinneganScanNumber: 5294
Data file 070708_BidirA_b1DV_10min_conj.msm

Monoisotopic mass of neutral peptide Mr(calc): 2576.0712
Fixed modifications: Carbamidomethyl (C)
Variable modifications: 
Y5     : Phospho (Y)
Ions Score: 42  Expect: 0.24  
Matches (Bold Red): 25/228 fragment ions using 44 most intense peaks

#	b	b⁺⁺	b*	b*⁺⁺	b⁰	b⁰⁺⁺	Seq.	y	y⁺⁺	y*	y*⁺⁺	y⁰	y⁰⁺⁺	#
1	72.0444	36.5258					A							22
2	187.0713	94.0393			169.0608	85.0340	D	2506.0414	1253.5243	2489.0149	1245.0111	2488.0308	1244.5191	21
3	274.1034	137.5553			256.0928	128.5500	S	2391.0145	1196.0109	2373.9879	1187.4976	2373.0039	1187.0056	20
4	403.1459	202.0766			385.1354	193.0713	E	2303.9824	1152.4949	2286.9559	1143.9816	2285.9719	1143.4896	19
5	646.1756	323.5914			628.1650	314.5862	Y	2174.9399	1087.9736	2157.9133	1079.4603	2156.9293	1078.9683	18
6	747.2233	374.1153			729.2127	365.1100	T	1931.9102	966.4587	1914.8837	957.9455	1913.8996	957.4535	17
7	862.2502	431.6287			844.2397	422.6235	D	1830.8625	915.9349	1813.8360	907.4216	1812.8520	906.9296	16
8	990.3452	495.6762	973.3186	487.1630	972.3346	486.6709	K	1715.8356	858.4214	1698.8090	849.9082	1697.8250	849.4161	15
9	1103.4292	552.2183	1086.4027	543.7050	1085.4187	543.2130	L	1587.7406	794.3739	1570.7141	785.8607	1569.7301	785.3687	14
10	1231.4878	616.2475	1214.4613	607.7343	1213.4773	607.2423	Q	1474.6566	737.8319	1457.6300	729.3186	1456.6460	728.8266	13
11	1368.5467	684.7770	1351.5202	676.2637	1350.5362	675.7717	H	1346.5980	673.8026	1329.5714	665.2894	1328.5874	664.7973	12
12	1531.6101	766.3087	1514.5835	757.7954	1513.5995	757.3034	Y	1209.5391	605.2732	1192.5125	596.7599	1191.5285	596.2679	11
13	1632.6577	816.8325	1615.6312	808.3192	1614.6472	807.8272	T	1046.4757	523.7415	1029.4492	515.2282	1028.4652	514.7362	10
14	1719.6898	860.3485	1702.6632	851.8352	1701.6792	851.3432	S	945.4281	473.2177	928.4015	464.7044	927.4175	464.2124	9
15	1776.7112	888.8593	1759.6847	880.3460	1758.7007	879.8540	G	858.3960	429.7017	841.3695	421.1884	840.3855	420.6964	8
16	1913.7701	957.3887	1896.7436	948.8754	1895.7596	948.3834	H	801.3746	401.1909	784.3480	392.6776	783.3640	392.1856	7
17	2044.8106	1022.9089	2027.7841	1014.3957	2026.8001	1013.9037	M	664.3157	332.6615	647.2891	324.1482	646.3051	323.6562	6
18	2145.8583	1073.4328	2128.8318	1064.9195	2127.8477	1064.4275	T	533.2752	267.1412	516.2486	258.6280	515.2646	258.1359	5
19	2242.9111	1121.9592	2225.8845	1113.4459	2224.9005	1112.9539	P	432.2275	216.6174	415.2010	208.1041			4
20	2299.9325	1150.4699	2282.9060	1141.9566	2281.9220	1141.4646	G	335.1747	168.0910	318.1482	159.5777			3
21	2430.9730	1215.9901	2413.9465	1207.4769	2412.9624	1206.9849	M	278.1533	139.5803	261.1267	131.0670			2
22							K	147.1128	74.0600	130.0863	65.5468			1

Error Distribution Error Distribution (ppm)

NCBI BLAST search of ADSEYTDKLQHYTSGHMTPGMK
(Parameters: blastp, nr protein database, expect=20000, no filter, PAM30)
Other BLAST web gateways

All matches to this query

Score Mr(calc): Delta Sequence

41.5 2576.0712 0.0040 ADSEYTDKLQHYTSGHMTPGMK

34.4 2576.0712 0.0040 ADSEYTDKLQHYTSGHMTPGMK

32.2 2576.0712 0.0040 ADSEYTDKLQHYTSGHMTPGMK

10.8 2576.0563 0.0190 HQRTPENYPNDSMFGNGKGYR

9.3 2576.0686 0.0066 GGFVLVHAGAGYHSESKAKEYK

8.3 2576.0563 0.0190 HQRTPENYPNDSMFGNGKGYR

8.2 2576.0344 0.0408 QETKSTDGQEPHSVVYDTSNGK

8.2 2576.0344 0.0408 QETKSTDGQEPHSVVYDTSNGK

7.9 2576.0946 -0.0193 MDHRVCPPLSHSESFGVPKGR

7.8 2576.1026 -0.0273 SHLANEMMMYHMKVSDDEYTK

Score	Mr(calc):	Delta	Sequence
41.5	2576.0712	0.0040	ADSEYTDKLQHYTSGHMTPGMK
34.4	2576.0712	0.0040	ADSEYTDKLQHYTSGHMTPGMK
32.2	2576.0712	0.0040	ADSEYTDKLQHYTSGHMTPGMK
10.8	2576.0563	0.0190	HQRTPENYPNDSMFGNGKGYR
9.3	2576.0686	0.0066	GGFVLVHAGAGYHSESKAKEYK
8.3	2576.0563	0.0190	HQRTPENYPNDSMFGNGKGYR
8.2	2576.0344	0.0408	QETKSTDGQEPHSVVYDTSNGK
8.2	2576.0344	0.0408	QETKSTDGQEPHSVVYDTSNGK
7.9	2576.0946	-0.0193	MDHRVCPPLSHSESFGVPKGR
7.8	2576.1026	-0.0273	SHLANEMMMYHMKVSDDEYTK

Mascot: http://www.matrixscience.com/