Mascot Search Results

Peptide View

MS/MS Fragmentation of GSHQISLDNPDYQQDFFPK
Found in ENSP00000275493, gene:EGFR -- Epidermal growth factor receptor precursor (EC 2.7.10.1) (Receptor tyrosine-protein

Match to Query 24015: 2314.991580 from(772.671136,3+)
Title: Elution from: 777.777 to 777.777 period: 060308_bidira_10min_4g10_ip2.raw experiment: 1 cycles: 1 precIntensity: 11311.0 FinneganScanNumber: 7464
Data file 060308_B2DW_bidirA_10min_conj.msm

Monoisotopic mass of neutral peptide Mr(calc): 2314.9895
Fixed modifications: Carbamidomethyl (C)
Variable modifications: 
Y12    : Phospho (Y)
Ions Score: 50  Expect: 0.029  
Matches (Bold Red): 41/200 fragment ions using 71 most intense peaks

#	b	b⁺⁺	b*	b*⁺⁺	b⁰	b⁰⁺⁺	Seq.	y	y⁺⁺	y*	y*⁺⁺	y⁰	y⁰⁺⁺	#
1	58.0287	29.5180					G							19
2	145.0608	73.0340			127.0502	64.0287	S	2258.9754	1129.9913	2241.9488	1121.4781	2240.9648	1120.9860	18
3	282.1197	141.5635			264.1091	132.5582	H	2171.9433	1086.4753	2154.9168	1077.9620	2153.9328	1077.4700	17
4	410.1783	205.5928	393.1517	197.0795	392.1677	196.5875	Q	2034.8844	1017.9459	2017.8579	1009.4326	2016.8739	1008.9406	16
5	523.2623	262.1348	506.2358	253.6215	505.2518	253.1295	I	1906.8259	953.9166	1889.7993	945.4033	1888.8153	944.9113	15
6	610.2943	305.6508	593.2678	297.1375	592.2838	296.6455	S	1793.7418	897.3745	1776.7152	888.8613	1775.7312	888.3693	14
7	723.3784	362.1928	706.3519	353.6796	705.3678	353.1876	L	1706.7098	853.8585	1689.6832	845.3452	1688.6992	844.8532	13
8	838.4053	419.7063	821.3788	411.1930	820.3948	410.7010	D	1593.6257	797.3165	1576.5992	788.8032	1575.6151	788.3112	12
9	952.4483	476.7278	935.4217	468.2145	934.4377	467.7225	N	1478.5988	739.8030	1461.5722	731.2897	1460.5882	730.7977	11
10	1049.5010	525.2542	1032.4745	516.7409	1031.4905	516.2489	P	1364.5558	682.7816	1347.5293	674.2683	1346.5453	673.7763	10
11	1164.5280	582.7676	1147.5014	574.2544	1146.5174	573.7623	D	1267.5031	634.2552	1250.4765	625.7419	1249.4925	625.2499	9
12	1407.5576	704.2825	1390.5311	695.7692	1389.5471	695.2772	Y	1152.4761	576.7417	1135.4496	568.2284	1134.4656	567.7364	8
13	1535.6162	768.3117	1518.5897	759.7985	1517.6056	759.3065	Q	909.4465	455.2269	892.4199	446.7136	891.4359	446.2216	7
14	1663.6748	832.3410	1646.6482	823.8278	1645.6642	823.3358	Q	781.3879	391.1976	764.3614	382.6843	763.3773	382.1923	6
15	1778.7017	889.8545	1761.6752	881.3412	1760.6912	880.8492	D	653.3293	327.1683	636.3028	318.6550	635.3188	318.1630	5
16	1925.7701	963.3887	1908.7436	954.8754	1907.7596	954.3834	F	538.3024	269.6548	521.2758	261.1416			4
17	2072.8385	1036.9229	2055.8120	1028.4096	2054.8280	1027.9176	F	391.2340	196.1206	374.2074	187.6073			3
18	2169.8913	1085.4493	2152.8648	1076.9360	2151.8807	1076.4440	P	244.1656	122.5864	227.1390	114.0731			2
19							K	147.1128	74.0600	130.0863	65.5468			1

Error Distribution Error Distribution (ppm)

NCBI BLAST search of GSHQISLDNPDYQQDFFPK
(Parameters: blastp, nr protein database, expect=20000, no filter, PAM30)
Other BLAST web gateways

All matches to this query

Score Mr(calc): Delta Sequence

50.2 2314.9895 0.0020 GSHQISLDNPDYQQDFFPK

10.8 2314.9540 0.0376 YRYGEILESEGSIRDLR

10.8 2314.9540 0.0376 YRYGEILESEGSIRDLR

10.8 2314.9540 0.0376 YRYGEILESEGSIRDLR

10.8 2314.9540 0.0376 YRYGEILESEGSIRDLR

10.4 2314.9875 0.0040 NILVNSNLVCKVSDFGMSR

10.4 2314.9875 0.0040 NILVNSNLVCKVSDFGMSR

9.9 2314.9594 0.0322 VSASEQGGGDPKSALNASDNLK

9.8 2314.9924 -0.0009 ETMKNEYKELNDAYTAAAR

9.0 2315.0219 -0.0303 NEPTTRSPVSSQQSLKNLR

Score	Mr(calc):	Delta	Sequence
50.2	2314.9895	0.0020	GSHQISLDNPDYQQDFFPK
10.8	2314.9540	0.0376	YRYGEILESEGSIRDLR
10.8	2314.9540	0.0376	YRYGEILESEGSIRDLR
10.8	2314.9540	0.0376	YRYGEILESEGSIRDLR
10.8	2314.9540	0.0376	YRYGEILESEGSIRDLR
10.4	2314.9875	0.0040	NILVNSNLVCKVSDFGMSR
10.4	2314.9875	0.0040	NILVNSNLVCKVSDFGMSR
9.9	2314.9594	0.0322	VSASEQGGGDPKSALNASDNLK
9.8	2314.9924	-0.0009	ETMKNEYKELNDAYTAAAR
9.0	2315.0219	-0.0303	NEPTTRSPVSSQQSLKNLR

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