Mascot Search Results

Peptide View

MS/MS Fragmentation of EHALLAYTLGVK
Found in ENSP00000330054, gene:EEF1A1 -- Elongation factor 1-alpha 1 (EF-1-alpha-1) (Elongation factor 1 A-1) (eEF1A-1) (El

Match to Query 9923: 1395.707106 from(698.860829,2+)
Title: Elution from: 777.777 to 777.777 period: 160308_bidirb2.raw experiment: 1 cycles: 1 precIntensity: 23339.0 FinneganScanNumber: 7077
Data file 160308_bidirb_B2DIC_10min_conj.msm

Monoisotopic mass of neutral peptide Mr(calc): 1395.6947
Fixed modifications: Carbamidomethyl (C)
Variable modifications: 
Y7     : Phospho (Y)
K12    : Lysine-15N2 (K_2)
Ions Score: 44  Expect: 0.044  
Matches (Bold Red): 21/102 fragment ions using 35 most intense peaks

#	b	b⁺⁺	b⁰	b⁰⁺⁺	Seq.	y	y⁺⁺	y*	y*⁺⁺	y⁰	y⁰⁺⁺	#
1	130.0499	65.5286	112.0393	56.5233	E							12
2	267.1088	134.0580	249.0982	125.0527	H	1267.6594	634.3333	1250.6328	625.8201	1249.6488	625.3281	11
3	338.1459	169.5766	320.1353	160.5713	A	1130.6005	565.8039	1113.5739	557.2906	1112.5899	556.7986	10
4	451.2299	226.1186	433.2194	217.1133	L	1059.5634	530.2853	1042.5368	521.7721	1041.5528	521.2800	9
5	564.3140	282.6606	546.3034	273.6554	L	946.4793	473.7433	929.4528	465.2300	928.4687	464.7380	8
6	635.3511	318.1792	617.3406	309.1739	A	833.3953	417.2013	816.3687	408.6880	815.3847	408.1960	7
7	878.3808	439.6940	860.3702	430.6887	Y	762.3581	381.6827	745.3316	373.1694	744.3476	372.6774	6
8	979.4284	490.2179	961.4179	481.2126	T	519.3285	260.1679	502.3019	251.6546	501.3179	251.1626	5
9	1092.5125	546.7599	1074.5019	537.7546	L	418.2808	209.6440	401.2543	201.1308			4
10	1149.5340	575.2706	1131.5234	566.2653	G	305.1968	153.1020	288.1702	144.5887			3
11	1248.6024	624.8048	1230.5918	615.7995	V	248.1753	124.5913	231.1487	116.0780			2
12					K	149.1069	75.0571	132.0803	66.5438			1

Error Distribution Error Distribution (ppm)

NCBI BLAST search of EHALLAYTLGVK
(Parameters: blastp, nr protein database, expect=20000, no filter, PAM30)
Other BLAST web gateways

All matches to this query

Score Mr(calc): Delta Sequence

43.9 1395.6947 0.0124 EHALLAYTLGVK

14.8 1395.7271 -0.0199 ISGNLATIQKLR

14.6 1395.7216 -0.0145 KANQLASQVEYK

14.6 1395.7216 -0.0145 KANQLASQVEYK

9.9 1395.6861 0.0210 HMTIHVPLDASR

9.0 1395.6852 0.0219 MGPFLTSQEKEK

7.9 1395.7213 -0.0142 RQRLPMPSPER

7.3 1395.7167 -0.0096 VSASPVLRLGSAR

7.0 1395.7279 -0.0208 TKKPVVIRASK

7.0 1395.7279 -0.0208 TKKPVVIRASK

Score	Mr(calc):	Delta	Sequence
43.9	1395.6947	0.0124	EHALLAYTLGVK
14.8	1395.7271	-0.0199	ISGNLATIQKLR
14.6	1395.7216	-0.0145	KANQLASQVEYK
14.6	1395.7216	-0.0145	KANQLASQVEYK
9.9	1395.6861	0.0210	HMTIHVPLDASR
9.0	1395.6852	0.0219	MGPFLTSQEKEK
7.9	1395.7213	-0.0142	RQRLPMPSPER
7.3	1395.7167	-0.0096	VSASPVLRLGSAR
7.0	1395.7279	-0.0208	TKKPVVIRASK
7.0	1395.7279	-0.0208	TKKPVVIRASK

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