Mascot Search Results

Peptide View

MS/MS Fragmentation of EHALLAYTLGVK
Found in ENSP00000330054, gene:EEF1A1 -- Elongation factor 1-alpha 1 (EF-1-alpha-1) (Elongation factor 1 A-1) (eEF1A-1) (El

Match to Query 13291: 1401.716280 from(701.865416,2+)
Title: Elution from: 777.777 to 777.777 period: 160308_bidir_b_10min_ptyr100_ip1.raw experiment: 1 cycles: 1 precIntensity: 84453.0 FinneganScanNumber: 6679
Data file 160308_bidirb_10min_conj.msm

Monoisotopic mass of neutral peptide Mr(calc): 1401.7148
Fixed modifications: Carbamidomethyl (C)
Variable modifications: 
Y7     : Phospho (Y)
K12    : Lysine-13C615N2 (K_8)
Ions Score: 47  Expect: 0.022  
Matches (Bold Red): 25/102 fragment ions using 48 most intense peaks

#	b	b⁺⁺	b⁰	b⁰⁺⁺	Seq.	y	y⁺⁺	y*	y*⁺⁺	y⁰	y⁰⁺⁺	#
1	130.0499	65.5286	112.0393	56.5233	E							12
2	267.1088	134.0580	249.0982	125.0527	H	1273.6795	637.3434	1256.6530	628.8301	1255.6690	628.3381	11
3	338.1459	169.5766	320.1353	160.5713	A	1136.6206	568.8139	1119.5941	560.3007	1118.6100	559.8087	10
4	451.2299	226.1186	433.2194	217.1133	L	1065.5835	533.2954	1048.5570	524.7821	1047.5729	524.2901	9
5	564.3140	282.6606	546.3034	273.6554	L	952.4994	476.7534	935.4729	468.2401	934.4889	467.7481	8
6	635.3511	318.1792	617.3406	309.1739	A	839.4154	420.2113	822.3888	411.6981	821.4048	411.2060	7
7	878.3808	439.6940	860.3702	430.6887	Y	768.3783	384.6928	751.3517	376.1795	750.3677	375.6875	6
8	979.4284	490.2179	961.4179	481.2126	T	525.3486	263.1779	508.3221	254.6647	507.3381	254.1727	5
9	1092.5125	546.7599	1074.5019	537.7546	L	424.3009	212.6541	407.2744	204.1408			4
10	1149.5340	575.2706	1131.5234	566.2653	G	311.2169	156.1121	294.1903	147.5988			3
11	1248.6024	624.8048	1230.5918	615.7995	V	254.1954	127.6013	237.1689	119.0881			2
12					K	155.1270	78.0671	138.1005	69.5539			1

Error Distribution Error Distribution (ppm)

NCBI BLAST search of EHALLAYTLGVK
(Parameters: blastp, nr protein database, expect=20000, no filter, PAM30)
Other BLAST web gateways

All matches to this query

Score Mr(calc): Delta Sequence

46.7 1401.7148 0.0015 EHALLAYTLGVK

13.8 1401.7077 0.0086 QHALLQEELRR

10.3 1401.6938 0.0225 ATFMEVQRTGSR

7.3 1401.6919 0.0244 FTNSLTTVGSNKK

7.1 1401.7033 0.0130 DPTHRAPQRGPR

7.0 1401.7148 0.0015 EHALLAYTLGVK

7.0 1401.7371 -0.0208 YSLSLQRENFK

5.7 1401.7375 -0.0212 FVGQMMEIKRK

4.4 1401.6909 0.0253 VISALADMQSKK

4.2 1401.7409 -0.0246 AMMTMAVLSKRK

Score	Mr(calc):	Delta	Sequence
46.7	1401.7148	0.0015	EHALLAYTLGVK
13.8	1401.7077	0.0086	QHALLQEELRR
10.3	1401.6938	0.0225	ATFMEVQRTGSR
7.3	1401.6919	0.0244	FTNSLTTVGSNKK
7.1	1401.7033	0.0130	DPTHRAPQRGPR
7.0	1401.7148	0.0015	EHALLAYTLGVK
7.0	1401.7371	-0.0208	YSLSLQRENFK
5.7	1401.7375	-0.0212	FVGQMMEIKRK
4.4	1401.6909	0.0253	VISALADMQSKK
4.2	1401.7409	-0.0246	AMMTMAVLSKRK

Mascot: http://www.matrixscience.com/