Mascot Search Results

Peptide View

MS/MS Fragmentation of AIIEKEYQPHVIVSTTGPNPFTTLTDRELEEYR
Found in ENSP00000348100, gene:ADD1 -- adducin 1 (alpha) isoform b [Source:RefSeq_peptide;Acc:NP_054908]

Match to Query 34314: 3925.919012 from(982.487029,4+)
Title: Elution from: 777.777 to 777.777 period: 230208_b2dw_b1fc_5min_ptyr100_ip2.raw experiment: 1 cycles: 1 precIntensity: 104129.0 FinneganScanNumber: 10165
Data file 230208_DWB2_b1Fc_5min_conj.msm

Monoisotopic mass of neutral peptide Mr(calc): 3925.9138
Fixed modifications: Carbamidomethyl (C)
Variable modifications: 
Y7     : Phospho (Y)
Ions Score: 45  Expect: 0.13  
Matches (Bold Red): 29/366 fragment ions using 35 most intense peaks

#	b	b⁺⁺	b*	b*⁺⁺	b⁰	b⁰⁺⁺	Seq.	y	y⁺⁺	y*	y*⁺⁺	y⁰	y⁰⁺⁺	#
1	72.0444	36.5258					A							33
2	185.1284	93.0679					I	3855.8840	1928.4456	3838.8575	1919.9324	3837.8735	1919.4404	32
3	298.2125	149.6099					I	3742.8000	1871.9036	3725.7734	1863.3903	3724.7894	1862.8983	31
4	427.2551	214.1312			409.2445	205.1259	E	3629.7159	1815.3616	3612.6893	1806.8483	3611.7053	1806.3563	30
5	555.3501	278.1787	538.3235	269.6654	537.3395	269.1734	K	3500.6733	1750.8403	3483.6468	1742.3270	3482.6627	1741.8350	29
6	684.3926	342.7000	667.3661	334.1867	666.3821	333.6947	E	3372.5783	1686.7928	3355.5518	1678.2795	3354.5678	1677.7875	28
7	927.4223	464.2148	910.3958	455.7015	909.4117	455.2095	Y	3243.5358	1622.2715	3226.5092	1613.7582	3225.5252	1613.2662	27
8	1055.4809	528.2441	1038.4543	519.7308	1037.4703	519.2388	Q	3000.5061	1500.7567	2983.4796	1492.2434	2982.4955	1491.7514	26
9	1152.5336	576.7705	1135.5071	568.2572	1134.5231	567.7652	P	2872.4475	1436.7274	2855.4210	1428.2141	2854.4370	1427.7221	25
10	1289.5926	645.2999	1272.5660	636.7866	1271.5820	636.2946	H	2775.3948	1388.2010	2758.3682	1379.6877	2757.3842	1379.1957	24
11	1388.6610	694.8341	1371.6344	686.3208	1370.6504	685.8288	V	2638.3359	1319.6716	2621.3093	1311.1583	2620.3253	1310.6663	23
12	1501.7450	751.3761	1484.7185	742.8629	1483.7345	742.3709	I	2539.2674	1270.1374	2522.2409	1261.6241	2521.2569	1261.1321	22
13	1600.8134	800.9104	1583.7869	792.3971	1582.8029	791.9051	V	2426.1834	1213.5953	2409.1568	1205.0821	2408.1728	1204.5900	21
14	1687.8455	844.4264	1670.8189	835.9131	1669.8349	835.4211	S	2327.1150	1164.0611	2310.0884	1155.5478	2309.1044	1155.0558	20
15	1788.8931	894.9502	1771.8666	886.4369	1770.8826	885.9449	T	2240.0829	1120.5451	2223.0564	1112.0318	2222.0724	1111.5398	19
16	1889.9408	945.4740	1872.9143	936.9608	1871.9303	936.4688	T	2139.0353	1070.0213	2122.0087	1061.5080	2121.0247	1061.0160	18
17	1946.9623	973.9848	1929.9357	965.4715	1928.9517	964.9795	G	2037.9876	1019.4974	2020.9610	1010.9842	2019.9770	1010.4921	17
18	2044.0150	1022.5112	2026.9885	1013.9979	2026.0045	1013.5059	P	1980.9661	990.9867	1963.9396	982.4734	1962.9556	981.9814	16
19	2158.0580	1079.5326	2141.0314	1071.0193	2140.0474	1070.5273	N	1883.9134	942.4603	1866.8868	933.9470	1865.9028	933.4550	15
20	2255.1107	1128.0590	2238.0842	1119.5457	2237.1002	1119.0537	P	1769.8704	885.4389	1752.8439	876.9256	1751.8599	876.4336	14
21	2402.1791	1201.5932	2385.1526	1193.0799	2384.1686	1192.5879	F	1672.8177	836.9125	1655.7911	828.3992	1654.8071	827.9072	13
22	2503.2268	1252.1170	2486.2003	1243.6038	2485.2163	1243.1118	T	1525.7493	763.3783	1508.7227	754.8650	1507.7387	754.3730	12
23	2604.2745	1302.6409	2587.2480	1294.1276	2586.2639	1293.6356	T	1424.7016	712.8544	1407.6750	704.3412	1406.6910	703.8491	11
24	2717.3586	1359.1829	2700.3320	1350.6696	2699.3480	1350.1776	L	1323.6539	662.3306	1306.6274	653.8173	1305.6433	653.3253	10
25	2818.4062	1409.7068	2801.3797	1401.1935	2800.3957	1400.7015	T	1210.5698	605.7886	1193.5433	597.2753	1192.5593	596.7833	9
26	2933.4332	1467.2202	2916.4066	1458.7070	2915.4226	1458.2149	D	1109.5222	555.2647	1092.4956	546.7514	1091.5116	546.2594	8
27	3089.5343	1545.2708	3072.5077	1536.7575	3071.5237	1536.2655	R	994.4952	497.7512	977.4687	489.2380	976.4847	488.7460	7
28	3218.5769	1609.7921	3201.5503	1601.2788	3200.5663	1600.7868	E	838.3941	419.7007	821.3676	411.1874	820.3835	410.6954	6
29	3331.6609	1666.3341	3314.6344	1657.8208	3313.6504	1657.3288	L	709.3515	355.1794	692.3250	346.6661	691.3410	346.1741	5
30	3460.7035	1730.8554	3443.6770	1722.3421	3442.6930	1721.8501	E	596.2675	298.6374	579.2409	290.1241	578.2569	289.6321	4
31	3589.7461	1795.3767	3572.7196	1786.8634	3571.7356	1786.3714	E	467.2249	234.1161	450.1983	225.6028	449.2143	225.1108	3
32	3752.8095	1876.9084	3735.7829	1868.3951	3734.7989	1867.9031	Y	338.1823	169.5948	321.1557	161.0815			2
33							R	175.1190	88.0631	158.0924	79.5498			1

Error Distribution Error Distribution (ppm)

NCBI BLAST search of AIIEKEYQPHVIVSTTGPNPFTTLTDRELEEYR
(Parameters: blastp, nr protein database, expect=20000, no filter, PAM30)
Other BLAST web gateways

All matches to this query

Score Mr(calc): Delta Sequence

45.0 3925.9138 0.0052 AIIEKEYQPHVIVSTTGPNPFTTLTDRELEEYR

19.8 3925.8486 0.0704 QKPVLQKYAELLVSQGVVNQPEYEEEISKYDK

19.8 3925.8486 0.0704 QKPVLQKYAELLVSQGVVNQPEYEEEISKYDK

19.0 3925.8716 0.0474 RPTVLEPIPMEAASSASSTREGQSWQPGAVATLPQR

18.4 3925.8486 0.0704 QKPVLQKYAELLVSQGVVNQPEYEEEISKYDK

18.4 3925.8716 0.0474 RPTVLEPIPMEAASSASSTREGQSWQPGAVATLPQR

14.3 3925.8486 0.0704 QKPVLQKYAELLVSQGVVNQPEYEEEISKYDK

14.3 3925.8486 0.0704 QKPVLQKYAELLVSQGVVNQPEYEEEISKYDK

13.7 3925.9681 -0.0491 KAFTTISTHLTVVIFYYAPFVYTYLRPRNLR

13.7 3925.9681 -0.0491 KAFTTISTHLTVVIFYYAPFVYTYLRPRNLR

Score	Mr(calc):	Delta	Sequence
45.0	3925.9138	0.0052	AIIEKEYQPHVIVSTTGPNPFTTLTDRELEEYR
19.8	3925.8486	0.0704	QKPVLQKYAELLVSQGVVNQPEYEEEISKYDK
19.8	3925.8486	0.0704	QKPVLQKYAELLVSQGVVNQPEYEEEISKYDK
19.0	3925.8716	0.0474	RPTVLEPIPMEAASSASSTREGQSWQPGAVATLPQR
18.4	3925.8486	0.0704	QKPVLQKYAELLVSQGVVNQPEYEEEISKYDK
18.4	3925.8716	0.0474	RPTVLEPIPMEAASSASSTREGQSWQPGAVATLPQR
14.3	3925.8486	0.0704	QKPVLQKYAELLVSQGVVNQPEYEEEISKYDK
14.3	3925.8486	0.0704	QKPVLQKYAELLVSQGVVNQPEYEEEISKYDK
13.7	3925.9681	-0.0491	KAFTTISTHLTVVIFYYAPFVYTYLRPRNLR
13.7	3925.9681	-0.0491	KAFTTISTHLTVVIFYYAPFVYTYLRPRNLR

Mascot: http://www.matrixscience.com/