Mascot Search Results

Peptide View

MS/MS Fragmentation of DSYVGDEAQSK
Found in ENSP00000290378, gene:ACTC1 -- Actin, alpha cardiac muscle 1 (Alpha-cardiac actin). [Source:Uniprot/SWISSPROT;Acc:

Match to Query 5861: 1277.494962 from(639.754757,2+)
Title: Elution from: 777.777 to 777.777 period: 021107_dwbidira_py100_ip2.raw experiment: 1 cycles: 1 precIntensity: 11961.0 FinneganScanNumber: 2530
Data file 021107_BidirA_B2DW_10min_conj_2.msm

Monoisotopic mass of neutral peptide Mr(calc): 1277.4812
Fixed modifications: Carbamidomethyl (C)
Variable modifications: 
Y3     : Phospho (Y)
Ions Score: 44  Expect: 0.015  
Matches (Bold Red): 22/102 fragment ions using 27 most intense peaks

#	b	b⁺⁺	b*	b*⁺⁺	b⁰	b⁰⁺⁺	Seq.	y	y⁺⁺	y*	y*⁺⁺	y⁰	y⁰⁺⁺	#
1	116.0342	58.5207			98.0237	49.5155	D							11
2	203.0662	102.0368			185.0557	93.0315	S	1163.4616	582.2344	1146.4351	573.7212	1145.4510	573.2292	10
3	446.0959	223.5516			428.0853	214.5463	Y	1076.4296	538.7184	1059.4030	530.2052	1058.4190	529.7131	9
4	545.1643	273.0858			527.1537	264.0805	V	833.3999	417.2036	816.3734	408.6903	815.3894	408.1983	8
5	602.1858	301.5965			584.1752	292.5912	G	734.3315	367.6694	717.3050	359.1561	716.3209	358.6641	7
6	717.2127	359.1100			699.2021	350.1047	D	677.3101	339.1587	660.2835	330.6454	659.2995	330.1534	6
7	846.2553	423.6313			828.2447	414.6260	E	562.2831	281.6452	545.2566	273.1319	544.2725	272.6399	5
8	917.2924	459.1498			899.2818	450.1446	A	433.2405	217.1239	416.2140	208.6106	415.2300	208.1186	4
9	1045.3510	523.1791	1028.3244	514.6659	1027.3404	514.1739	Q	362.2034	181.6053	345.1769	173.0921	344.1928	172.6001	3
10	1132.3830	566.6951	1115.3565	558.1819	1114.3725	557.6899	S	234.1448	117.5761	217.1183	109.0628	216.1343	108.5708	2
11							K	147.1128	74.0600	130.0863	65.5468			1

Error Distribution Error Distribution (ppm)

NCBI BLAST search of DSYVGDEAQSK
(Parameters: blastp, nr protein database, expect=20000, no filter, PAM30)
Other BLAST web gateways

All matches to this query

Score Mr(calc): Delta Sequence

44.4 1277.4812 0.0137 DSYVGDEAQSK

21.7 1277.4812 0.0137 DSYVGDEAQSK

15.4 1277.5181 -0.0231 SKENSSKVTK

5.6 1277.5064 -0.0114 TYTEEELNAK

3.6 1277.4957 -0.0008 ASNGRYVCMK

2.6 1277.5151 -0.0202 GSASPMAGSGAKR

1.5 1277.5151 -0.0202 GSASPMAGSGAKR

1.1 1277.5181 -0.0231 SKENSSKVTK

0.8 1277.4974 -0.0024 SGTEAASRVAR

0.8 1277.4974 -0.0024 SGTEAASRVAR

Score	Mr(calc):	Delta	Sequence
44.4	1277.4812	0.0137	DSYVGDEAQSK
21.7	1277.4812	0.0137	DSYVGDEAQSK
15.4	1277.5181	-0.0231	SKENSSKVTK
5.6	1277.5064	-0.0114	TYTEEELNAK
3.6	1277.4957	-0.0008	ASNGRYVCMK
2.6	1277.5151	-0.0202	GSASPMAGSGAKR
1.5	1277.5151	-0.0202	GSASPMAGSGAKR
1.1	1277.5181	-0.0231	SKENSSKVTK
0.8	1277.4974	-0.0024	SGTEAASRVAR
0.8	1277.4974	-0.0024	SGTEAASRVAR

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