Mascot Search Results

Peptide View

MS/MS Fragmentation of TQTPPVSPAPQPTEERLPSSPVYEDAASFK
Found in ENSP00000317189, gene:CTTN -- Src substrate cortactin (Amplaxin) (Oncogene EMS1). [Source:Uniprot/SWISSPROT;Acc:Q1

Match to Query 33929: 3385.515444 from(1129.512424,3+)
Title: Elution from: 777.777 to 777.777 period: 100308_b2dw_b1fc_5min_4g10_ip1.raw experiment: 1 cycles: 1 precIntensity: 179060.0 FinneganScanNumber: 8696
Data file 100308_DWB2_b1Fc_5min_conj.msm

Monoisotopic mass of neutral peptide Mr(calc): 3385.5156
Fixed modifications: Carbamidomethyl (C)
Variable modifications: 
T1     : Phospho (ST), with neutral loss 97.9769
Y23    : Phospho (Y)
Ions Score: 73  Expect: 0.00021  
Matches (Bold Red): 67/342 fragment ions using 102 most intense peaks

#	b	b⁺⁺	b*	b*⁺⁺	b⁰	b⁰⁺⁺	Seq.	y	y⁺⁺	y*	y*⁺⁺	y⁰	y⁰⁺⁺	#
1	84.0444	42.5258			66.0338	33.5205	T							30
2	212.1030	106.5551	195.0764	98.0418	194.0924	97.5498	Q	3205.5089	1603.2581	3188.4823	1594.7448	3187.4983	1594.2528	29
3	313.1506	157.0790	296.1241	148.5657	295.1401	148.0737	T	3077.4503	1539.2288	3060.4237	1530.7155	3059.4397	1530.2235	28
4	410.2034	205.6053	393.1769	197.0921	392.1928	196.6001	P	2976.4026	1488.7049	2959.3760	1480.1917	2958.3920	1479.6997	27
5	507.2562	254.1317	490.2296	245.6184	489.2456	245.1264	P	2879.3498	1440.1786	2862.3233	1431.6653	2861.3393	1431.1733	26
6	606.3246	303.6659	589.2980	295.1527	588.3140	294.6606	V	2782.2971	1391.6522	2765.2705	1383.1389	2764.2865	1382.6469	25
7	693.3566	347.1819	676.3301	338.6687	675.3460	338.1767	S	2683.2287	1342.1180	2666.2021	1333.6047	2665.2181	1333.1127	24
8	790.4094	395.7083	773.3828	387.1950	772.3988	386.7030	P	2596.1966	1298.6020	2579.1701	1290.0887	2578.1861	1289.5967	23
9	861.4465	431.2269	844.4199	422.7136	843.4359	422.2216	A	2499.1439	1250.0756	2482.1173	1241.5623	2481.1333	1241.0703	22
10	958.4992	479.7533	941.4727	471.2400	940.4887	470.7480	P	2428.1068	1214.5570	2411.0802	1206.0437	2410.0962	1205.5517	21
11	1086.5578	543.7825	1069.5313	535.2693	1068.5472	534.7773	Q	2331.0540	1166.0306	2314.0275	1157.5174	2313.0434	1157.0254	20
12	1183.6106	592.3089	1166.5840	583.7957	1165.6000	583.3036	P	2202.9954	1102.0014	2185.9689	1093.4881	2184.9849	1092.9961	19
13	1284.6583	642.8328	1267.6317	634.3195	1266.6477	633.8275	T	2105.9427	1053.4750	2088.9161	1044.9617	2087.9321	1044.4697	18
14	1413.7008	707.3541	1396.6743	698.8408	1395.6903	698.3488	E	2004.8950	1002.9511	1987.8684	994.4379	1986.8844	993.9458	17
15	1542.7434	771.8754	1525.7169	763.3621	1524.7329	762.8701	E	1875.8524	938.4298	1858.8258	929.9166	1857.8418	929.4246	16
16	1698.8445	849.9259	1681.8180	841.4126	1680.8340	840.9206	R	1746.8098	873.9085	1729.7833	865.3953	1728.7992	864.9033	15
17	1811.9286	906.4679	1794.9021	897.9547	1793.9180	897.4627	L	1590.7087	795.8580	1573.6821	787.3447	1572.6981	786.8527	14
18	1908.9814	954.9943	1891.9548	946.4810	1890.9708	945.9890	P	1477.6246	739.3160	1460.5981	730.8027	1459.6141	730.3107	13
19	1996.0134	998.5103	1978.9868	989.9971	1978.0028	989.5051	S	1380.5719	690.7896	1363.5453	682.2763	1362.5613	681.7843	12
20	2083.0454	1042.0263	2066.0189	1033.5131	2065.0349	1033.0211	S	1293.5398	647.2736	1276.5133	638.7603	1275.5293	638.2683	11
21	2180.0982	1090.5527	2163.0716	1082.0395	2162.0876	1081.5474	P	1206.5078	603.7575	1189.4813	595.2443	1188.4973	594.7523	10
22	2279.1666	1140.0869	2262.1400	1131.5737	2261.1560	1131.0817	V	1109.4551	555.2312	1092.4285	546.7179	1091.4445	546.2259	9
23	2522.1962	1261.6018	2505.1697	1253.0885	2504.1857	1252.5965	Y	1010.3866	505.6970	993.3601	497.1837	992.3761	496.6917	8
24	2651.2388	1326.1231	2634.2123	1317.6098	2633.2283	1317.1178	E	767.3570	384.1821	750.3304	375.6689	749.3464	375.1769	7
25	2766.2658	1383.6365	2749.2392	1375.1233	2748.2552	1374.6312	D	638.3144	319.6608	621.2879	311.1476	620.3038	310.6556	6
26	2837.3029	1419.1551	2820.2763	1410.6418	2819.2923	1410.1498	A	523.2875	262.1474	506.2609	253.6341	505.2769	253.1421	5
27	2908.3400	1454.6736	2891.3134	1446.1604	2890.3294	1445.6684	A	452.2504	226.6288	435.2238	218.1155	434.2398	217.6235	4
28	2995.3720	1498.1897	2978.3455	1489.6764	2977.3615	1489.1844	S	381.2132	191.1103	364.1867	182.5970	363.2027	182.1050	3
29	3142.4404	1571.7239	3125.4139	1563.2106	3124.4299	1562.7186	F	294.1812	147.5942	277.1547	139.0810			2
30							K	147.1128	74.0600	130.0863	65.5468			1

Error Distribution Error Distribution (ppm)

NCBI BLAST search of TQTPPVSPAPQPTEERLPSSPVYEDAASFK
(Parameters: blastp, nr protein database, expect=20000, no filter, PAM30)
Other BLAST web gateways

All matches to this query

Score Mr(calc): Delta Sequence

72.8 3385.5156 -0.0001 TQTPPVSPAPQPTEERLPSSPVYEDAASFK

71.7 3385.5156 -0.0001 TQTPPVSPAPQPTEERLPSSPVYEDAASFK

56.6 3385.5156 -0.0001 TQTPPVSPAPQPTEERLPSSPVYEDAASFK

25.9 3385.5156 -0.0001 TQTPPVSPAPQPTEERLPSSPVYEDAASFK

16.8 3385.5156 -0.0001 TQTPPVSPAPQPTEERLPSSPVYEDAASFK

15.5 3385.5171 -0.0017 RPYLGPALLLTPRDRASGTQGASEDNSGGGGK

13.8 3385.5156 -0.0001 TQTPPVSPAPQPTEERLPSSPVYEDAASFK

12.8 3385.5417 -0.0263 VEQLEKTFQEEEQHLQGLEIAQGEKDLK

11.1 3385.5171 -0.0017 RPYLGPALLLTPRDRASGTQGASEDNSGGGGK

11.0 3385.5039 0.0116 EYSSQSPTSSSPEKVLLTELLASNDEWVK

Score	Mr(calc):	Delta	Sequence
72.8	3385.5156	-0.0001	TQTPPVSPAPQPTEERLPSSPVYEDAASFK
71.7	3385.5156	-0.0001	TQTPPVSPAPQPTEERLPSSPVYEDAASFK
56.6	3385.5156	-0.0001	TQTPPVSPAPQPTEERLPSSPVYEDAASFK
25.9	3385.5156	-0.0001	TQTPPVSPAPQPTEERLPSSPVYEDAASFK
16.8	3385.5156	-0.0001	TQTPPVSPAPQPTEERLPSSPVYEDAASFK
15.5	3385.5171	-0.0017	RPYLGPALLLTPRDRASGTQGASEDNSGGGGK
13.8	3385.5156	-0.0001	TQTPPVSPAPQPTEERLPSSPVYEDAASFK
12.8	3385.5417	-0.0263	VEQLEKTFQEEEQHLQGLEIAQGEKDLK
11.1	3385.5171	-0.0017	RPYLGPALLLTPRDRASGTQGASEDNSGGGGK
11.0	3385.5039	0.0116	EYSSQSPTSSSPEKVLLTELLASNDEWVK

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