Mascot Search Results

Peptide View

MS/MS Fragmentation of TQTPPVSPAPQPTEERLPSSPVYEDAASFK
Found in ENSP00000317189, gene:CTTN -- Src substrate cortactin (Amplaxin) (Oncogene EMS1). [Source:Uniprot/SWISSPROT;Acc:Q1

Match to Query 8066: 3483.515262 from(1162.179030,3+)
Title: Elution from: 777.777 to 777.777 period: 091207_bidira_10min_2e6_4g10.raw experiment: 1 cycles: 1 precIntensity: 45041.0 FinneganScanNumber: 6963
Data file 091207_BidirA_b1_10min_conj.msm

Monoisotopic mass of neutral peptide Mr(calc): 3483.5044
Fixed modifications: Carbamidomethyl (C)
Variable modifications: 
T3     : Phospho (ST), with neutral loss 97.9769
S7     : Phospho (ST), with neutral loss 97.9769
R16    : Arginine-13C615N4 (R_10)
Y23    : Phospho (Y)
K30    : Lysine-13C615N2 (K_8)
Ions Score: 55  Expect: 0.022  
Matches (Bold Red): 57/342 fragment ions using 111 most intense peaks

#	b	b⁺⁺	b*	b*⁺⁺	b⁰	b⁰⁺⁺	Seq.	y	y⁺⁺	y*	y*⁺⁺	y⁰	y⁰⁺⁺	#
1	102.0550	51.5311			84.0444	42.5258	T							30
2	230.1135	115.5604	213.0870	107.0471	212.1030	106.5551	Q	3187.5102	1594.2587	3170.4837	1585.7455	3169.4996	1585.2535	29
3	313.1506	157.0790	296.1241	148.5657	295.1401	148.0737	T	3059.4516	1530.2294	3042.4251	1521.7162	3041.4411	1521.2242	28
4	410.2034	205.6053	393.1769	197.0921	392.1928	196.6001	P	2976.4145	1488.7109	2959.3880	1480.1976	2958.4039	1479.7056	27
5	507.2562	254.1317	490.2296	245.6184	489.2456	245.1264	P	2879.3618	1440.1845	2862.3352	1431.6712	2861.3512	1431.1792	26
6	606.3246	303.6659	589.2980	295.1527	588.3140	294.6606	V	2782.3090	1391.6581	2765.2824	1383.1449	2764.2984	1382.6529	25
7	675.3460	338.1767	658.3195	329.6634	657.3355	329.1714	S	2683.2406	1342.1239	2666.2140	1333.6107	2665.2300	1333.1186	24
8	772.3988	386.7030	755.3722	378.1898	754.3882	377.6978	P	2614.2191	1307.6132	2597.1926	1299.0999	2596.2086	1298.6079	23
9	843.4359	422.2216	826.4094	413.7083	825.4253	413.2163	A	2517.1664	1259.0868	2500.1398	1250.5735	2499.1558	1250.0815	22
10	940.4887	470.7480	923.4621	462.2347	922.4781	461.7427	P	2446.1293	1223.5683	2429.1027	1215.0550	2428.1187	1214.5630	21
11	1068.5472	534.7773	1051.5207	526.2640	1050.5367	525.7720	Q	2349.0765	1175.0419	2332.0499	1166.5286	2331.0659	1166.0366	20
12	1165.6000	583.3036	1148.5735	574.7904	1147.5894	574.2984	P	2221.0179	1111.0126	2203.9914	1102.4993	2203.0074	1102.0073	19
13	1266.6477	633.8275	1249.6211	625.3142	1248.6371	624.8222	T	2123.9652	1062.4862	2106.9386	1053.9729	2105.9546	1053.4809	18
14	1395.6903	698.3488	1378.6637	689.8355	1377.6797	689.3435	E	2022.9175	1011.9624	2005.8909	1003.4491	2004.9069	1002.9571	17
15	1524.7329	762.8701	1507.7063	754.3568	1506.7223	753.8648	E	1893.8749	947.4411	1876.8483	938.9278	1875.8643	938.4358	16
16	1690.8423	845.9248	1673.8157	837.4115	1672.8317	836.9195	R	1764.8323	882.9198	1747.8057	874.4065	1746.8217	873.9145	15
17	1803.9263	902.4668	1786.8998	893.9535	1785.9158	893.4615	L	1598.7229	799.8651	1581.6964	791.3518	1580.7123	790.8598	14
18	1900.9791	950.9932	1883.9525	942.4799	1882.9685	941.9879	P	1485.6388	743.3231	1468.6123	734.8098	1467.6283	734.3178	13
19	1988.0111	994.5092	1970.9846	985.9959	1970.0005	985.5039	S	1388.5861	694.7967	1371.5595	686.2834	1370.5755	685.7914	12
20	2075.0431	1038.0252	2058.0166	1029.5119	2057.0326	1029.0199	S	1301.5541	651.2807	1284.5275	642.7674	1283.5435	642.2754	11
21	2172.0959	1086.5516	2155.0693	1078.0383	2154.0853	1077.5463	P	1214.5220	607.7646	1197.4955	599.2514	1196.5115	598.7594	10
22	2271.1643	1136.0858	2254.1378	1127.5725	2253.1537	1127.0805	V	1117.4693	559.2383	1100.4427	550.7250	1099.4587	550.2330	9
23	2514.1940	1257.6006	2497.1674	1249.0873	2496.1834	1248.5953	Y	1018.4009	509.7041	1001.3743	501.1908	1000.3903	500.6988	8
24	2643.2366	1322.1219	2626.2100	1313.6086	2625.2260	1313.1166	E	775.3712	388.1892	758.3446	379.6760	757.3606	379.1840	7
25	2758.2635	1379.6354	2741.2369	1371.1221	2740.2529	1370.6301	D	646.3286	323.6679	629.3021	315.1547	628.3180	314.6627	6
26	2829.3006	1415.1539	2812.2741	1406.6407	2811.2900	1406.1487	A	531.3017	266.1545	514.2751	257.6412	513.2911	257.1492	5
27	2900.3377	1450.6725	2883.3112	1442.1592	2882.3271	1441.6672	A	460.2646	230.6359	443.2380	222.1226	442.2540	221.6306	4
28	2987.3697	1494.1885	2970.3432	1485.6752	2969.3592	1485.1832	S	389.2274	195.1174	372.2009	186.6041	371.2169	186.1121	3
29	3134.4382	1567.7227	3117.4116	1559.2094	3116.4276	1558.7174	F	302.1954	151.6013	285.1689	143.0881			2
30							K	155.1270	78.0671	138.1005	69.5539			1

Error Distribution Error Distribution (ppm)

NCBI BLAST search of TQTPPVSPAPQPTEERLPSSPVYEDAASFK
(Parameters: blastp, nr protein database, expect=20000, no filter, PAM30)
Other BLAST web gateways

All matches to this query

Score Mr(calc): Delta Sequence

54.9 3483.5044 0.0109 TQTPPVSPAPQPTEERLPSSPVYEDAASFK

34.4 3483.5044 0.0109 TQTPPVSPAPQPTEERLPSSPVYEDAASFK

25.6 3483.5044 0.0109 TQTPPVSPAPQPTEERLPSSPVYEDAASFK

11.4 3483.5127 0.0025 QSQMLEMQVELSSMKDRATELQEQLSSEK

10.4 3483.4522 0.0630 NKGYLPGQGPFVAAFSSSNLGDVSPNILGPR

8.7 3483.4522 0.0630 NKGYLPGQGPFVAAFSSSNLGDVSPNILGPR

8.3 3483.5053 0.0100 NDIYSVSRQIEIRGLESAVIYGSLPPGTK

8.0 3483.5116 0.0036 LRSPAVLAPAVLTPHSGSETPRGTHPGTGHR

7.6 3483.5230 -0.0077 NPVTSTYSKMSLQLYQVDSRTYLLDFR

7.5 3483.5326 -0.0173 ECGKTFSLHGYLNQHQKIHTGMKPYECK

Score	Mr(calc):	Delta	Sequence
54.9	3483.5044	0.0109	TQTPPVSPAPQPTEERLPSSPVYEDAASFK
34.4	3483.5044	0.0109	TQTPPVSPAPQPTEERLPSSPVYEDAASFK
25.6	3483.5044	0.0109	TQTPPVSPAPQPTEERLPSSPVYEDAASFK
11.4	3483.5127	0.0025	QSQMLEMQVELSSMKDRATELQEQLSSEK
10.4	3483.4522	0.0630	NKGYLPGQGPFVAAFSSSNLGDVSPNILGPR
8.7	3483.4522	0.0630	NKGYLPGQGPFVAAFSSSNLGDVSPNILGPR
8.3	3483.5053	0.0100	NDIYSVSRQIEIRGLESAVIYGSLPPGTK
8.0	3483.5116	0.0036	LRSPAVLAPAVLTPHSGSETPRGTHPGTGHR
7.6	3483.5230	-0.0077	NPVTSTYSKMSLQLYQVDSRTYLLDFR
7.5	3483.5326	-0.0173	ECGKTFSLHGYLNQHQKIHTGMKPYECK

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