Mascot Search Results

Peptide View

MS/MS Fragmentation of TQTPPVSPAPQPTEERLPSSPVYEDAASFK
Found in ENSP00000317189, gene:CTTN -- Src substrate cortactin (Amplaxin) (Oncogene EMS1). [Source:Uniprot/SWISSPROT;Acc:Q1

Match to Query 34032: 3466.498413 from(1156.506747,3+)
Title: Elution from: 777.777 to 777.777 period: 100308_b2dw_b1fc_5min_4g10_ip1.raw experiment: 1 cycles: 1 precIntensity: 34912.0 FinneganScanNumber: 9231
Data file 100308_DWB2_b1Fc_5min_conj.msm

Monoisotopic mass of neutral peptide Mr(calc): 3466.4659
Fixed modifications: Carbamidomethyl (C)
Variable modifications: 
T3     : Phospho (ST), with neutral loss 97.9769
S7     : Phospho (ST), with neutral loss 97.9769
Q11    : Deamidation (NQ)
Y23    : Phospho (Y)
Ions Score: 48  Expect: 0.062  
Matches (Bold Red): 55/342 fragment ions using 107 most intense peaks

#	b	b⁺⁺	b*	b*⁺⁺	b⁰	b⁰⁺⁺	Seq.	y	y⁺⁺	y*	y*⁺⁺	y⁰	y⁰⁺⁺	#
1	102.0550	51.5311			84.0444	42.5258	T							30
2	230.1135	115.5604	213.0870	107.0471	212.1030	106.5551	Q	3170.4717	1585.7395	3153.4452	1577.2262	3152.4612	1576.7342	29
3	313.1506	157.0790	296.1241	148.5657	295.1401	148.0737	T	3042.4131	1521.7102	3025.3866	1513.1969	3024.4026	1512.7049	28
4	410.2034	205.6053	393.1769	197.0921	392.1928	196.6001	P	2959.3760	1480.1917	2942.3495	1471.6784	2941.3655	1471.1864	27
5	507.2562	254.1317	490.2296	245.6184	489.2456	245.1264	P	2862.3233	1431.6653	2845.2967	1423.1520	2844.3127	1422.6600	26
6	606.3246	303.6659	589.2980	295.1527	588.3140	294.6606	V	2765.2705	1383.1389	2748.2440	1374.6256	2747.2599	1374.1336	25
7	675.3460	338.1767	658.3195	329.6634	657.3355	329.1714	S	2666.2021	1333.6047	2649.1756	1325.0914	2648.1915	1324.5994	24
8	772.3988	386.7030	755.3722	378.1898	754.3882	377.6978	P	2597.1806	1299.0940	2580.1541	1290.5807	2579.1701	1290.0887	23
9	843.4359	422.2216	826.4094	413.7083	825.4253	413.2163	A	2500.1279	1250.5676	2483.1013	1242.0543	2482.1173	1241.5623	22
10	940.4887	470.7480	923.4621	462.2347	922.4781	461.7427	P	2429.0908	1215.0490	2412.0642	1206.5358	2411.0802	1206.0437	21
11	1069.5313	535.2693	1052.5047	526.7560	1051.5207	526.2640	Q	2332.0380	1166.5226	2315.0115	1158.0094	2314.0274	1157.5174	20
12	1166.5840	583.7956	1149.5575	575.2824	1148.5734	574.7904	P	2202.9954	1102.0014	2185.9689	1093.4881	2184.9849	1092.9961	19
13	1267.6317	634.3195	1250.6051	625.8062	1249.6211	625.3142	T	2105.9427	1053.4750	2088.9161	1044.9617	2087.9321	1044.4697	18
14	1396.6743	698.8408	1379.6477	690.3275	1378.6637	689.8355	E	2004.8950	1002.9511	1987.8684	994.4379	1986.8844	993.9458	17
15	1525.7169	763.3621	1508.6903	754.8488	1507.7063	754.3568	E	1875.8524	938.4298	1858.8258	929.9166	1857.8418	929.4246	16
16	1681.8180	841.4126	1664.7914	832.8994	1663.8074	832.4073	R	1746.8098	873.9085	1729.7833	865.3953	1728.7992	864.9033	15
17	1794.9020	897.9547	1777.8755	889.4414	1776.8915	888.9494	L	1590.7087	795.8580	1573.6821	787.3447	1572.6981	786.8527	14
18	1891.9548	946.4810	1874.9283	937.9678	1873.9442	937.4758	P	1477.6246	739.3160	1460.5981	730.8027	1459.6141	730.3107	13
19	1978.9868	989.9971	1961.9603	981.4838	1960.9763	980.9918	S	1380.5719	690.7896	1363.5453	682.2763	1362.5613	681.7843	12
20	2066.0189	1033.5131	2048.9923	1024.9998	2048.0083	1024.5078	S	1293.5398	647.2736	1276.5133	638.7603	1275.5293	638.2683	11
21	2163.0716	1082.0394	2146.0451	1073.5262	2145.0611	1073.0342	P	1206.5078	603.7575	1189.4813	595.2443	1188.4973	594.7523	10
22	2262.1400	1131.5737	2245.1135	1123.0604	2244.1295	1122.5684	V	1109.4551	555.2312	1092.4285	546.7179	1091.4445	546.2259	9
23	2505.1697	1253.0885	2488.1431	1244.5752	2487.1591	1244.0832	Y	1010.3866	505.6970	993.3601	497.1837	992.3761	496.6917	8
24	2634.2123	1317.6098	2617.1857	1309.0965	2616.2017	1308.6045	E	767.3570	384.1821	750.3304	375.6689	749.3464	375.1769	7
25	2749.2392	1375.1232	2732.2127	1366.6100	2731.2286	1366.1180	D	638.3144	319.6608	621.2879	311.1476	620.3038	310.6556	6
26	2820.2763	1410.6418	2803.2498	1402.1285	2802.2658	1401.6365	A	523.2875	262.1474	506.2609	253.6341	505.2769	253.1421	5
27	2891.3134	1446.1604	2874.2869	1437.6471	2873.3029	1437.1551	A	452.2504	226.6288	435.2238	218.1155	434.2398	217.6235	4
28	2978.3455	1489.6764	2961.3189	1481.1631	2960.3349	1480.6711	S	381.2132	191.1103	364.1867	182.5970	363.2027	182.1050	3
29	3125.4139	1563.2106	3108.3873	1554.6973	3107.4033	1554.2053	F	294.1812	147.5942	277.1547	139.0810			2
30							K	147.1128	74.0600	130.0863	65.5468			1

Error Distribution Error Distribution (ppm)

NCBI BLAST search of TQTPPVSPAPQPTEERLPSSPVYEDAASFK
(Parameters: blastp, nr protein database, expect=20000, no filter, PAM30)
Other BLAST web gateways

All matches to this query

Score Mr(calc): Delta Sequence

48.1 3466.4659 0.0325 TQTPPVSPAPQPTEERLPSSPVYEDAASFK

47.1 3466.4659 0.0325 TQTPPVSPAPQPTEERLPSSPVYEDAASFK

26.3 3466.4659 0.0325 TQTPPVSPAPQPTEERLPSSPVYEDAASFK

25.8 3466.4659 0.0325 TQTPPVSPAPQPTEERLPSSPVYEDAASFK

21.8 3466.4659 0.0325 TQTPPVSPAPQPTEERLPSSPVYEDAASFK

17.3 3466.4659 0.0325 TQTPPVSPAPQPTEERLPSSPVYEDAASFK

11.8 3466.4780 0.0204 KTIGECSMSLRTLSTQEMDYSLDITPPSK

11.6 3466.4585 0.0399 INLSLSALGNVIAALAGNRSTHIPYRDSK

11.1 3466.4850 0.0135 DASPVGIKGFNTLPSTEISMYTLSRDPLK

8.3 3466.5214 -0.0230 LTTLNSMHSHFILSDDGTVGKYGNEMKLR

Score	Mr(calc):	Delta	Sequence
48.1	3466.4659	0.0325	TQTPPVSPAPQPTEERLPSSPVYEDAASFK
47.1	3466.4659	0.0325	TQTPPVSPAPQPTEERLPSSPVYEDAASFK
26.3	3466.4659	0.0325	TQTPPVSPAPQPTEERLPSSPVYEDAASFK
25.8	3466.4659	0.0325	TQTPPVSPAPQPTEERLPSSPVYEDAASFK
21.8	3466.4659	0.0325	TQTPPVSPAPQPTEERLPSSPVYEDAASFK
17.3	3466.4659	0.0325	TQTPPVSPAPQPTEERLPSSPVYEDAASFK
11.8	3466.4780	0.0204	KTIGECSMSLRTLSTQEMDYSLDITPPSK
11.6	3466.4585	0.0399	INLSLSALGNVIAALAGNRSTHIPYRDSK
11.1	3466.4850	0.0135	DASPVGIKGFNTLPSTEISMYTLSRDPLK
8.3	3466.5214	-0.0230	LTTLNSMHSHFILSDDGTVGKYGNEMKLR

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